NF-κB Reporter (Luc) - CHO-K1 Recombinant Cell Line

Catalog #: 60622
Size: 2 vials

An NF-κB luciferase reporter construct is stably integrated into the genome of CHO-K1 cells. The firefly luciferase gene is controlled by the NF-κB response element located upstream of the TATA promoter. Following activation by stimulants, endogenous NF-κB transcription factors bind to the DNA response elements to induce transcription of the luciferase gene.

The NF-κB-luciferase / CHO-K1 cell line is suitable for monitoring the activity of NF-κB
transcription factor through luminescence readout.). This cell line responds to human cytokine
IL-1β, responds moderately to human TNF, and does not respond to human IFN-λ (2 μg/ml).
Reducing the amount of serum during incubation period may increase the sensitivity to
cytokines. Since CHO-K1 cells do not express endogenous human proteins, this cell line
provides an excellent platform to enable exogenous expression of a protein of interest to study
its downstream effect on NF-κB signaling.

NF-κB cell line, NFkappaB cell line, luciferase reporter, NF-kB cell line, NFkB cell line
Host Cell line:
Mycoplasma Testing:
This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza, Cat. #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, Cat. #LT07-518) was used as a positive control.
Growth Media:

Thaw Medium 3 (BPS Bioscience, #60186): Ham’s F-12 medium (Hyclone #SH30526.01) supplemented with 10% FBS (Life technologies #26140-079), 1% Penicillin/Streptomycin (Hyclone #SV30010.01).   

Growth Medium 3D (BPS Bioscience, #79539): Thaw Medium 3 (BPS Bioscience, #60186) plus 1 mg/ml G418 (Thermo Fisher, #11811031). 

Aqueous solution containing DMSO
Instructions for use:
See product datasheet
Storage / Stability:
Store in liquid nitrogen immediately upon receipt.

1. Delude, R.L., (1994) CD14-mediated Translocation of Nuclear Factor-kB Induced by Lipopolysaccharide Does Not Require Tyrosine Kinase Activity. J. Biol. Chem. 269: 22253
2. Railo, A., (2008) Wnt-11 signaling leads to down-regulation of the Wnt/betacatenin, JNK/AP-1 and NF-kappaB pathways and promotes viability in the CHO-K1 cells. Exp Cell Res. 314: 2389-99
3. Murphy, S.H., (2011) Tumor suppressor protein (p)53, is a regulator of NF-κB repression by the glucocorticoid receptor. Proc. Natl. Acad. Sci. USA 108: 17117-17122


License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Scientific Category:
Cell Signaling Pathway
Product Type:
Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.

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