ISRE Reporter – HEK293 Recombinant Cell Line (JAK pathway)

Catalog #: 60510
Size: 2 vials
$1,295
Background:
The JAK (Janus kinase) /STAT (Signal Transducer and Activator of Transcription) pathway is activated by various cytokines and growth factors and plays a critical role in cell growth, hematopoiesis, and immune response. In mammals, there are four JAKs (JAK1, JAK2, JAK3 and TYK2) and seven STAT proteins. Binding of Interferon alpha (IFNα) to its receptor leads to the activation of JAK1 and TYK2, which in turn phosphorylate and activate STAT1 and STAT2. The phosphorylated STAT1 and 2 form a heterodimer and bind to IRF9/p48, forming a protein complex known as ISGF3. This complex translocates to the nucleus and binds to the ISRE (Interferon Stimulated Response Element) in the promoter region thereby promoting transcription of interferon-inducible genes.
Description:

The ISRE Reporter – HEK293 Cell Line is designed for monitoring the activity of the JAK/STAT signaling pathway. The ISRE Reporter – HEK293 Cell Line contains the firefly luciferase gene under the control of ISRE stably integrated into HEK293 cells.

Materials Required But Not Provided:

• Human Interferon Alpha A (IFN) (R&D Systems #11100-1)
• JAK Inhibitor I (Pyridone 6) (EMD Millipore #420099). Prepare stock solution in DMSO.
• Assay Medium: Thaw Medium 1 (BPS Cat. #60187)
Growth Medium 1B (BPS Cat. #79531)
• 96-well tissue culture plate or 96-well tissue culture-treated white clear-bottom assay plate 
ONE-Step™ Luciferase Assay System (BPS Cat. #60690)
• Luminometer 

Synonym(s):
JAK reporter, STAT reporter, ISRE cell line
Supplied As:
Each vial contains ~1.5 X 106 cells in 1 ml of 10% DMSO.
Biological Activity:
This cell line is validated for the response to stimulation with interferon Alpha A and to treatment with JAK inhibitor.
Host Cell line:
HEK293 cells
Mycoplasma Testing:
The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.
Growth Media:

Thaw Medium 1 (BPS Cat. #60187): MEM medium (Hyclone #SH30024.01) supplemented with 10% FBS (Invitrogen #26140-079), 1% non-essential amino acids (Hyclone #SH30238.01), 1 mM Na pyruvate (Hyclone #SH30239.01), 1% Penicillin/Streptomycin (Hyclone SV30010.01).

Growth Medium 1B (BPS Cat. #79531): Thaw Medium 1 plus 400 μg/ml of Geneticin (Life Technologies #11811031).

Format:
Aqueous solution containing DMSO.
Instructions for use:
See data sheet for detailed culturing and assay protocol.
Storage / Stability:

Store in liquid nitrogen immediately upon receipt.

Application(s):
• Monitor IFNα-induced activity and the JAK/STAT pathway activity.
• Screen for activators or inhibitors of the JAK/STAT signaling pathway.
Reference(s):

1. Hebenstreit D et al. (2005) JAK/STAT-dependent gene regulation by cytokines. Drug News Perspect 18(4): 243–249.

2. Pedranzini L et al. (2006) Pyridone 6, a pan-Janus-activated kinase inhibitor, induces growth inhibition of multiple myeloma cells. Cancer Res. 66(19):9714-9721. 

Notes:

License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Cell Signaling Pathway
Product Type:
Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

60510.pdf