CRE/CREB Reporter (Luc) – HEK293 Recombinant Cell Line (cAMP / PKA Signaling

Catalog #: 60515
Size: 2 vials
The main role of the cAMP response element, or CRE, is mediating the effects of Protein Kinase A (PKA) by way of transcription. It is the main binding site of cAMP response element binding protein (CREB) and is responsible for its activation. CRE is the target of many extracellular and intracellular signaling pathways, including cAMP, calcium, GPCR (G-protein coupled receptors) and neurotrophins. The cAMP/PKA signaling pathway is critical to numerous life processes in living organisms. In the cAMP/PKA signaling pathway, CREB is activated via phosphorylation of PKA and binds to CRE with a general motif of 5’-TGACGTCA-3’. Since CRE is a modulator of the cAMP/PKA signaling pathway, it allows the effects of various inhibitors to be studied.
The cAMP/PKA Signaling Pathway CRE/CREB Reporter (Luc) – HEK293 Cell Line is designed for monitoring the activity of the cAMP/ PKA signaling pathway. The cAMP/PKA Signaling Pathway CRE/CREB Reporter (Luc) – HEK293 Cell Line contains a firefly luciferase gene under the control of multimerized cAMP response element (CRE) stably integrated into HEK293 cells. Elevation of the intracellular cAMP level activates cAMP response element binding protein (CREB) to bind CRE and induces the expression of luciferase.
Materials Required But Not Provided:

• Forskolin (LC Laboratories #F-9929), 10 mM in DMSO: activator of cAMP expression 
• H-89 (Enzo Life Sciences # BML-EI196-0005) 10 mM in DMSO: inhibitor of cAMP/PKA pathway
• Assay medium: Thaw Medium 1 (BPS Cat. #60187)
Growth Medium 1F (BPS Cat. #79540)
• 96-well tissue culture treated white clear-bottom assay plate (Corning #3610)
ONE-Step™ Luciferase Assay System (BPS, Cat. #60690)
• Luminometer

CRE cell line, CREB cell line, luciferase reporter
Supplied As:
Each vial contains ~1.5 X 106 cells in 1 ml of 10% DMSO.
Biological Activity:
This cell line is validated for response to stimulation by Forskolin and to the treatment with an inhibitor of the cAMP/PKA signaling pathway.
Host Cell line:
HEK293 cells
Mycoplasma Testing:
The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.
Growth Media:

Thaw Medium 1 (BPS, #60187): MEM medium (Hyclone, #SH30024.01) supplemented with 10% FBS (Invitrogen, #26140-079), 1% non-essential amino acids (Hyclone, #SH30238.01), 1 mM Na pyruvate (Hyclone, #SH30239.01), 1% Penicillin/Streptomycin (Hyclone, #SV30010.01).

Growth Medium 1F (BPS, #79540): Thaw Medium 1 (BPS, #60187) plus 100 µg/ml Hygromycin B (Thermo Fisher, #10687010).

Aqueous solution containing DMSO.
Instructions for use:
See data sheet for detailed culturing and assay protocol.
Storage / Stability:

Store in liquid nitrogen immediately upon receipt.

• Monitor cAMP/PKA signaling pathway activity.
• Screen for activators or inhibitors of the cAMP/PKA signaling pathway.

1. Montminy, M.R. et al. (1987) Binding of a nuclear protein to the cyclic-AMP response element of the somatostatin gene. Nature 328(6126): 175-178.
2. Fan Chung, K. (2006) Phosphodiesterase inhibitors in airways disease. Eur. J. Pharmacol. 533(1-3): 110-117.
3. Malik, R. et al. (2008) Cloning, stable expression of human phosphodiesterase 7A and development of an assay for screening of PDE7 selective inhibitors. Appl. Microbiol. Biotechnol. 77(5): 1167-1173.


License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Avoid freeze/thaw cycles
Scientific Category:
Cell Signaling Pathway
Product Type:
Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.

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