CD27 CHO-K1 Stable Recombinant Cell Line

Catalog #: 60624
Size: 2 vials
CD27 is a member of the tumor necrosis factor (TNF) receptor superfamily, which includes the T cell co-stimulatory receptors OX40, 4-1BB and herpesvirus entry mediator (HVEM). CD27 is expressed on various types of T cells, B cells and a subset of natural killer cells. It activates NF- kB and MAPK/JNK signaling upon interaction with its TNF-like ligand, CD70, which is expressed by numerous tumor cells. Adaptor proteins TRAF2 and TRAF5 can also stimulate CD27 signaling. Activation of CD27 leads to lymphoid proliferation, differentiation, apoptosis, and the induction of long-term memory. The CD27/CD70 pathway is a key target for the development of treatments for cancer and inflammatory diseases.

CHO-K1 cell line expressing full length human CD27 (TNFRSF7). Expression is confirmed by real time qPCR, Western blot, and flow cytometry.

Screening for antibodies recognizing CD27 and screening antibodies for binding affinities. Since
CHO-K1 cells do not express endogenous CD27, this cell line serves as an excellent model for
studying changes in CD27-mediated signaling pathways, such as NF-kB, MAPK and

CD70 antigen, Tumor Necrosis Factor Ligand Superfamily Member 7, TNFSF7, CD27 Ligand, Ki-24 antigen, CD27-L, CD27LG,
Supplied As:
Each vial contains ~2 X 106 cells in 1 ml of 10% DMSO.
Host Cell line:
Mycoplasma Testing:
This cell line has been screened using the MycoAlertâ„¢ Mycoplasma Detection Kit (Lonza, Cat. #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, Cat. #LT07-518) was used as a positive control.
Growth Media:

Thaw Medium 3 (BPS Bioscience, #60186): Ham’s F-12 medium (Hyclone #SH30526.01) supplemented with 10% FBS (Life technologies #26140-079), 1% Penicillin/Streptomycin (Hyclone #SV30010.01).   

Growth Medium 3E (BPS Bioscience, #79553): Thaw Medium 3 (BPS Bioscience, #60186) plus 100 µg/ml Hygromycin B (Thermo Fisher, #10687010).

Aqueous solution containing DMSO
Instructions for use:
See datasheet
Storage / Stability:
Store in liquid nitrogen immediately upon receipt.

1. Akiba H (1998) CD27, a member of the tumor necrosis factor receptor superfamily, activates NF-kB and stress-activated protein kinase/ c-Jun N-terminal kinase via TRAF2, TRAF5, and NF-kB inducing kinase. J. Biol. Chem.  273: 13353

2. Yamamoto H (1998) NF-kB activation in CD27 signaling: Involvement of TNF receptor-associated factor in its signaling and identification of functional region of CD27. J. Immunology  161: 4753-4759


License Disclosure:Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Scientific Category:
Immunotherapy/Cell Surface Receptor
Product Type:
Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.

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