CD137L (4-1BBL) CHO-K1 Recombinant Cell Line

Catalog #: 60523
Size: 2 vials
CD137L (4-1BBL; TNLG5A), a part of the tumor necrosis factor (ligand) superfamily (TNFSF9), is a type II glycoprotein that expresses on various antigen-presenting cells. It interacts with CD137 (4-1BB) on T cells following TCR stimulation to promote T cell expansion and cytokine production.
Recombinant CHO-K1 cell constitutively expressing full-length human CD137L (NP_003802.1). Surface expression is confirmed by flow cytometry.
CD137L, 4-1BBL
Host Cell line:
Chinese Hamster Ovary Cells. Adherent epithelial cells.
Mycoplasma Testing:
This cell line has been screened using the MycoAlertâ„¢ Mycoplasma Detection Kit (catalogue #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (catalogue #LT07-518) was used as a positive control.
Growth Media:

Thaw Medium 3 (BPS Bioscience, #60186): Ham’s F-12 medium (Hyclone #SH30526.01) supplemented with 10% FBS (Life technologies #26140-079), 1% Penicillin/Streptomycin (Hyclone #SV30010.01).   

Growth Medium 3D (BPS Bioscience, #79539): Thaw Medium 3 (BPS Bioscience, #60186) plus 1 mg/ml G418 (Thermo Fisher, #11811031). 

Each vial contains 2 x 10^6 cells in 1 ml of 10% DMSO in FBS.
Storage / Stability:
Store in liquid nitrogen immediately upon receipt.
This cell line is useful to stimulate human CD137 (4-1BB) on immune cells, and for use in binding assays for CD137.
Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.
Scientific Category:
Product Type:
Cell Line
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