ADCC Bioassay Effector Cell F variant (Low Affinity) - Jurkat Recombinant Cell Line

Catalog #: 60540
Size: 2 vials
Antibody-dependent cell-mediated cytotoxicity (ADCC) is an immune defense mechanism involving an effector cell lysing a target cell on which antibodies have bound to specific antigens on the target cell membrane.

The typical ADCC involves activation of natural killer (NK) cells by antibodies. NK cells express Fc receptors, mostly CD16 or FcγRIIIa (CD16a), on its cell surface. These Fc receptors recognize and bind to the Fc portion of an antibody, such as IgG, which has bound to the surface of a pathogen-infected target cell. Once the Fc receptor binds to the Fc region of IgG, the Natural Killer cell releases cytokines such as IFN-γ and cytotoxic molecules that attack the pathogen-infected target cell.

The human FcγRIIIa displays a dimorphism in the position of residue 158. One allele (V158) encodes a higher Fc affinity receptor variant with a valine at amino acid residue 158, and the other (F158) encodes a lower Fc affinity receptor variant having a phenylalanine at amino acid residue 158.

Assay Principle:

Recombinant Jurkat T cell expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of human FcγRIIIa, low affinity (F158) variant and Fcγ chain.
Materials Required But Not Provided:

Materials Required But Not Supplied for Cell Culture

· Thaw Medium 2 (BPS Cat. #60184)

· Growth Medium 2A (BPS Cat. #60190)

Materials Required But Not Supplied for Cellular Assay

·  Assay Medium 2A (BPS Cat. #79621)

·  Anti-CD20, BPS #71209

·  96-well tissue culture-treated white clear-bottom assay plate

·  One-Step luciferase assay system (BPS Bioscience # 60690)

·  Luminometer

Antibody-dependent cell-mediated cytotoxicity
Mycoplasma Testing:
The cell line has been screened using the PCR-based Venor®GeM Mycoplasma Detection kit (Sigma-Aldrich) to confirm the absence of Mycoplasma species.
Growth Media:

Thaw Medium 2 (BPS Cat. #60184): RPMI1640 medium (Life Technologies #A10491-01) supplemented with 10% FBS (Life Technologies #26140-079), 1% Penicillin/Streptomycin (Hyclone #SV30010.01). 

Growth Medium 2A (BPS Cat. #60190): Thaw Medium 2 (BPS Cat. #60184) plus 1 mg/ml of Geneticin (Life Technologies #11811031) and 200 μg/ml of Hygromycin B (Hyclone #SV30070.01).

Each vial contains ~2 x 106 cells in 1 ml of 10% DMSO
Storage / Stability:
Immediately upon receipt, store in liquid nitrogen.
Characterize the Fc effector function of antibodies and measure ADCC activity in cellular assays.

License Disclosure:Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Scientific Category:
Product Type:
Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.

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