PDE7A/CRE Reporter - HEK293 Recombinant Cell Line

Catalog #: 60413
Size: 2 vials
$7,500
Species: human
Genbank #: NM_002603
Description:
The PDE7A/CRE Reporter-HEK293 line contains the firefly luciferase gene under the control of CRE as well as a constitutive expression construct for human PDE7A (phosphodiesterase 7A, accession number NM_002603), both stably integrated into HEK293 cells. The luciferase expression level from the CRE reporter is used to monitor the activity of PDE7A in the cells.
Synonym(s):
PDE7, phosphodiesterase 7, CRE reporter
Supplied As:
Each vial contains ~ X 106 cells in 1 ml of 10% DMSO.
Biological Activity:
The cell line is validated for the induction of the expression of luciferase reporter by an inhibitor of PDE7A.
Host Cell line:
HEK293 cells
Mycoplasma Testing:
The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma Aldrich) to confirm the absence of Mycoplasma species.
Instructions for use:
See data sheet for detailed culturing and assay protocol.
Storage / Stability:

Store in liquid nitrogen immediately upon receipt.

Application(s):
•Monitor Thuman PDE7A activity
•Screen for activators or inhibitors of PDE7A
Reference(s):

1. Malik, R. et al. (2008) Cloning, stable expression of human phosphodiesterase 7A and development of an assay for screening of PDE7 selective inhibitors. Appl.               Microbiol. Biotechnol. 77 (5): 1167-1173.

2.  Fan Chung, K. (2006) Phosphodiesterase inhibitors in airways disease. Eur. J. Pharmacol. 533(1-3):110-117.

Notes:

Contact us for pricing.

License Disclosure:Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
PDE
Product Type:
Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

60413.pdf