IDO2 - HEK293 Recombinant Cell Line (Human)

Catalog #: 60533
Size: 2 vials
$8,000
Species: Human
Genbank #: NM_194294
Background:
L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells.The L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp. Additionally, the released degradation products of L-Trp have immunosuppressive functions. Indoleamine 2,3-dioxygenases (IDO1 & IDO2), two of the rate limiting enzymes in this pathway, are upregulated in many tumorsand provide cancer cells with an avenue for immune evasion.
Description:
Recombinant HEK293 cell line expressing tetracycline-inducible human indoleamine 2,3-dioxygenase (IDO2), Genbank accession number NM_194294.
Synonym(s):
Indoleamine 2,3-dioxygenase 2, IDO2, IDO
Supplied As:
Each vial contains ~1.5 X 10^6 cells in 1 ml of 10% DMSO
Mycoplasma Testing:
The cell line has been screened using the PCR-based Venor®GeM Mycoplasma Detection kit (Sigma-Aldrich) to confirm the absence of Mycoplasma species.
Instructions for use:
See data sheet for detailed culturing and assay protocol
Storage / Stability:
Immediately upon receipt, store vials in liquid nitrogen.
Application(s):
Monitor IDO2 pathway activity and screen for activators or inhibitors of IDO2 in a cellular context
Reference(s):
1. Metz, R., et al., Int. Immunol. 2014; 26: 357–367.

2. Fatokun, A., et al., Amino Acids 2013; 45: 1319-1329.

Notes:

Contact us for pricing.

License Disclosure:Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Immunotherapy
Product Type:
Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

60533.pdf