IDO1-HEK293 Recombinant Cell Line (Human)

Catalog #: 60532
Size: 2 vials
Species: Human
Genbank #: NM_002164
L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. Indoleamine 2,3- dioxygenases (IDO1 & IDO2), two of the rate limiting enzymes in this pathway, are upregulated in many tumors, providing cancer cells with an avenue for immune evasion.

Stable Recombinant HEK293 IDO1 cell line expressing tetracycline-inducible human indoleamine 2,3-dioxygenase (IDO1) Genbank accession number NM_002164.

Materials Required But Not Provided:

•  Thaw Medium 1 (BPS Cat. #60187)
•  Growth Medium 1D (BPS Cat. #79536)
•  Geneticin (Sigma, #G8168) and  Blasticidin S (Sigma (#15205)
•  Doxycycline (MP Biomedicals #0219504401) 
•  PBS 
•  0.05% Trypsin EDTA 
•  6.1 N Trichloroacetic acid (Sigma #T0699) 
•  Acetic acid (Sigma #320099)  
IDO1   Cellular   Activity   QuickDetect™ Supplements   (BPS   Cat.   #62000)Note: other formulations can be used, but significant optimization may be required. 

Indoleamine 2,3-dioxygenase 1, IDO
Supplied As:
Each vial contains ~1.5 X 106 cells in 1 ml of 10% DMSO
Mycoplasma Testing:
The IDO1 cell line has been screened using the PCR-based VenorĀ®GeM Mycoplasma Detection kit (Sigma-Aldrich) to confirm the absence of Mycoplasma species.
Growth Media:

Thaw Medium 1 (BPS Cat. #60187): MEM medium (Hyclone #SH30024.01) supplemented with 10% FBS (Invitrogen #26140-079), 1% non-essential amino acids (Hyclone #SH30238.01), 1 mM Na pyruvate (Hyclone #SH30239.01), 1% Penicillin/Streptomycin (Hyclone SV30010.01).

Growth Medium 1D (BPS Cat. #79536): Thaw Medium 1 (BPS Cat. #60187) plus 600 µg/ml of Geneticin (Life Technologies #11811031), and 5 µg/ml of Blasticidin (Life Technologies # R21001) to ensure the recombinant expression plasmid is maintained.

Instructions for use:
See data sheet for detailed culturing and assay protocol
Storage / Stability:
Immediately upon receipt, store vials in liquid nitrogen.
Monitor IDO1 pathway activity. Screen for activators or inhibitors of IDO1 in a cellular context

1. Yue, E., et al., J. Med. Chem. 2009; 52: 7364–7367.
2. Liu, X., et al., Blood. 2010; 115(17): 3520-3530.


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License Disclosure:Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Avoid freeze/thaw cycles.
Scientific Category:
Product Type:
Cell Line
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