CFTR – HEK 293 Recombinant Cell Line

Catalog #: 60506
Size: 2 vials
Species: Human
Genbank #: P13569
Cystic fibrosis transmembrane conductance regulator (CFTR) is a protein that in humans is encoded by the CFTR gene. CFTR is an ABC transporter-class ion channel that transports chloride and thiocyanate ions across epithelial cell membranes. Mutations of the CFTR gene affect functioning of the chloride ion channels in these cell membranes, leading to cystic fibrosis. It is characterized by the triad of chronic bronchopulmonary disease (with recurrent respiratory infections), pancreatic insufficiency (which leads to malabsorption and growth retardation) and elevated sweat electrolytes.
The CFTR-HEK293 Cell Line stably expresses full length, human cystic fibrosis transmembrane conductance regulator (CFTR) protein (GenBank # P13569). A synthetic codon-optimized DNA sequence encoding human CFTR protein with C-terminal Streptavidin-Binding Peptide (SBP) tag is stably integrated in 293HEK-Trex cells.
CFTR, cystic fibrosis transmembrane conductance regulator
Supplied As:
Each vial contains ~2 X 106 cells in 1 ml of Sigma Freezing Medium (Cat. # C-6164).
Biological Activity:
The expression of CFTR is confirmed by Western blotting and flow cytometry (FACS).
Host Cell line:
HEK293 cells
Instructions for use:
See cell line data sheet for detailed culturing and assay protocol.
Storage / Stability:

Store in liquid nitrogen immediately upon receipt. Cells are stable after a minimum of seven continuous passages. Upon first use, amplify the cells in culture and make several frozen aliquots for future use.

•Drug compound screening for activators or inhibitors of CFTR
•Functional assays for CFTR
•Efficient antigen for mouse immunization
1. Gadsby, D.C., et al. Nature 440 (7083): 477–483 (2006).
2. Hillier, L.W., et al. Nature 424:157-164 (2003).
3. McCann, C. M., et al. BioTechniques 38 (6):945–952 (2005).

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License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Avoid freeze/thaw cycles
Scientific Category:
ion channel
Product Type:
Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.

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