TDO Cell-Based Assay Kit

Catalog #: 72033
Size: 100 reactions
$550
Background:
L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L-Trp that have immunosuppressive functions. Tryptophan 2,3 dioxygenase (TDO), one of the rate limiting enzymes in this pathway, is upregulated in many tumors, providing cancer cells with an avenue for immune evasion.
Description:

The human TDO Cell-Based Assay Kit is designed to monitor the activity of exogenously expressed human TDO in cultured cells.

Synonym(s):
Tryptophan 2,3-dioxygenase, TDO2, TDO-2, TDO
Supplied As:
The kit contains a transfection-ready expression vector for full length human TDO (hTDO). Upon expression, hTDO can convert L-Trp to Kyn, which gets released and is detected in the assay medium. The kit also includes specialized assay medium.
Format:

COMPONENTS:

Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Application(s):
Screen for inhibitors of hTDO enzyme in a cellular context.
Reference(s):
1. Yue, E., et al., J. Med. Chem. 2009; 52: 7364–7367.
2. Liu, X., et al., Blood. 2010; 115(17): 3520-3530.
Warning(s):
Avoid freeze/thaw cycles.
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

72033.pdf