IDO1 Cell-Based Assay Kit

Catalog #: 72031
Size: 100 reactions
$550
Background:
L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of LTrp that have immunosuppressive functions. Indoleamine 2,3-dioxygenases (IDO1 & IDO2), two of the rate limiting enzymes in this pathway, are upregulated in many tumors, providing cancer cells with an avenue for immune evasion.
Description:

The human IDO1 Cell-Based Assay Kit is designed to monitor the activity of exogenously expressed human IDO1 in cultured cells.

Synonym(s):
Indoleamine 2,3-dioxygenase 1, IDO, IDO2, IDO Assay IDO1 Assay
Supplied As:
The kit contains a transfection-ready expression vector for full length human IDO1 (hIDO1). Upon expression, hIDO1 catalyzes L-Trp conversion to Kyn, which gets released and is detected in the assay medium. The kit also includes specialized assay medium.
Format:

COMPONENTS:

Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Application(s):
Screen for inhibitors of hIDO1 enzyme in a cellular context. Note: The expression vector is designed for use in transient transfection protocols. It is NOT SUITABLE for transformation and amplification in bacteria.
Reference(s):

1. Yue, E., et al., J. Med. Chem. 2009; 52: 7364–7367.
2. Liu, X., et al., Blood. 2010; 115(17): 3520-3530.

Warning(s):
Avoid freeze/thaw cycles.
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

72031.pdf