IDO1 Cell-Based Assay Kit

Catalog #: 72031
Size: 100 reactions
L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis in mammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of LTrp that have immunosuppressive functions. Indoleamine 2,3-dioxygenases (IDO1 & IDO2), two of the rate limiting enzymes in this pathway, are upregulated in many tumors, providing cancer cells with an avenue for immune evasion.

The human IDO1 Cell-Based Assay Kit is designed to monitor the activity of exogenously expressed human IDO1 in cultured cells.

Indoleamine 2,3-dioxygenase 1, IDO, IDO2, IDO Assay IDO1 Assay
Supplied As:
The kit contains a transfection-ready expression vector for full length human IDO1 (hIDO1). Upon expression, hIDO1 catalyzes L-Trp conversion to Kyn, which gets released and is detected in the assay medium. The kit also includes specialized assay medium.


Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Screen for inhibitors of hIDO1 enzyme in a cellular context. Note: The expression vector is designed for use in transient transfection protocols. It is NOT SUITABLE for transformation and amplification in bacteria.

1. Yue, E., et al., J. Med. Chem. 2009; 52: 7364–7367.
2. Liu, X., et al., Blood. 2010; 115(17): 3520-3530.

Avoid freeze/thaw cycles.
Product Type:
Assay Kit
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