CECR2 Inhibitor Screening Assay Kit

Catalog #

32611

$1,025 *

Size: 384 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The CECR2 Inhibitor Screening Assay Kit is designed to measure the inhibition of CECR2 binding to its substrate. The key to the CECR2 Inhibitor Screening Assay Kit is the highly specific binding of the CECR2 bromodomain to the acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing CECR2 and an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.

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Synonyms

CECR2, cat eye syndrome chromosome region, candidate 2, activity assay kit

Product Info

Storage and Usage

Citations

Assay Kit Format

AlphaLISA®

Supplied As

Kit comes in a convenient AlphaLISA® format, with biotinylated histone peptide substrate, assay buffer, detection buffer and purified CECR2 (Cat Eye Syndrome Chromosome Region 2) bromodomain to perform a total of 384 enzyme reactions.

Format

COMPONENTS:

UniProt #

Q9BXF3

References

Lee S-K, et al., Mol. Cells 2012, 34(1):85-91.

Storage/Stability

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See data sheet for detailed protocol.

Applications

Useful for the study of bromodomain binding assays, screening bromodomain inhibitors and selectivity profiling.

Shipping Temperature

dry ice

Contraindications

Only limited amounts of DMSO can be included, as it has been shown to disrupt BRD-ligand interaction. Avoid green and blue dyes that absorb light in the AlphaScreen® signal emission range (520-620 nm), such as Trypan Blue.

Warnings

Avoid freeze/thaw cycles

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Bromodomain

Regulatory Status

Research Use Only

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CECR2 Inhibitor Screening Assay Kit

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