CDK5/p35, GST-tag Recombinant

Catalog #

40095

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant full-length human CDK5 (59 kDa) and human p35 (60 kDa), both with N-terminal GST-tags, were co-expressed in Sf9 cells using a baculovirus expression system. The CDK5 gene accession number is NM_004935 and p35 gene accession number is NM_003885.

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Synonyms

Cyclin-Dependent Kinase 5, Cell Devision Kinase 5, CDK5, p35, Cyclin-Dependent Kinase 5 Regulatory Subunit 1, CDK5R1, CDK-5

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Product Data Gallery

CDK5/p35 Activity

4-20% SDS-PAGE Coomassie Staining

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥60%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 20% glycerol and 3 mM DTT.

108 kDa (complex)

Amino Acids

full length

Specific Activity

180 pmol/min/µg

Genbank #

NM_004935 (CDK5), NM_003885 (p35)

UniProt #

Q00535 (CDK5), Q15078 (p35)

Tag(s)

N-terminal GST-tag

Background

CDK5 is a member of the Cyclin-Dependent Kinase family that is most abundant in the mammalian brain. Active form of CDK5, which has also been called neuronal cdc2-like kinase, is a heterodimer of CDK5 and a 25 kDa protein which is derived proteolytically from a 35 kDa brain and neuron-specific protein and is essential for the kinase activity of CDK5 (1). CDK5 has emerged as a crucial regulator of neuronal migration in the developing central nervous system. CDK5 phosphorylates a diverse list of substrates, implicating it in the regulation of a range of cellular processes - from adhesion and motility, to synaptic plasticity and drug addiction (2).

References

1. Tang, D. et al. Prog Cell Cycle Res. 1996,2:205-16.
2. Dhavan, R. et al. Nat Rev Mol Cell Biol. 2001 Oct,2(10):749-59.

Storage/Stability

>6 months at -80°C.

Assay Conditions

Assay was performed in Kinase buffer containing 0.2 mM DTT using Histone 1 substrate (0.2 mg/ml) and 20 mM ATP. Reaction was performed at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo® reagent (Promega).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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Datasheets/Protocols

40095_Lot121101.pdf

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CDK5/p35, GST-tag Recombinant

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