Complex of human CDK5, GenBank Accession No.NM_004935, a.a. 1-292(end) with N-terminal GST-tag, MW=60 kDa, and p25, GenBank Accession No.NM_003885, a.a. 99-307(end), with N-terminal GST-tag, MW=50 kDa. Coexpressed in a Baculovirus-infected Sf9 cell expression system.
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 4 mM glutathione, 0.04% Tween-20, and 20% glycerol
MW
60 kDa (CDK5), 50 kDa (p25)
Amino Acids
CDK5 1–292 (end), p25 99–307 (end)
Specific Activity
312 pmol/min/μg
Genbank #
NM_004935, NM_003885
UniProt #
Q00535 (CDK5), Q15078 (p35)
Tag(s)
N-terminal GST-tag
Background
CDK5 is a member of the Cyclin-Dependent Kinase family that is most abundant in the mammalian brain. Active form of CDK5, which has also been called neuronal cdc2-like kinase, is a heterodimer of CDK5 and a 25 kDa protein which is derived proteolytically from a 35 kDa brain and neuron-specific protein and is essential for the kinase activity of CDK5 (1). CDK5 has emerged as a crucial regulator of neuronal migration in the developing central nervous system. CDK5 phosphorylates a diverse list of substrates, implicating it in the regulation of a range of cellular processes - from adhesion and motility, to synaptic plasticity and drug addiction (2).
References
1. Tang, D. et al: Cyclin-dependent kinase 5 (Cdk5) and neuron-specific Cdk5 activators. Prog Cell Cycle Res. 1996;2:205-16. 2. Dhavan, R. et al: A decade of CDK5. Nat Rev Mol Cell Biol. 2001 Oct;2(10):749-59.
Assay was done in a Kinase buffer using Histone H1 (BPS cat. #52043) as a substrate with 20 μM ATP at 30°C for 30 min. The amount of ATP transferred was calculated using Kinase-Glo® reagent (Promega).
Instructions for Use
Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
Applications
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
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