Complex of human CDK1 (Cyclin Dependent Kinase 1) and human Cyclin A2. The two constructs are: recombinant human CDK1, full length encompassing amino acids 2-297 (end) and recombinant human Cyclin A2, full length encompassing amino acids 2-432 (end). Both constructs contain an N-terminal GST-Tag. A short peptide linker placed between GST and the protein includes a PreScission protease cleavage site. The two proteins were co-expressed in Sf9 insect cells and spontaneously formed a complex, which was affinity purified.
●
Synonyms
CDK1: Cyclin-dependent kinase 1, CDK-1, Cell division protein kinase 1, cell division control protein 2 homolog, CDC2, CDC28A, p34 protein kinas, P34CDC2 Cyclin A2: CCNA2, CCN1, CCNA
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 3 mM DTT
MW
CDK1: 60 kDa; CyclinA2: 75 kDa
Amino Acids
CDK1: 2-297(end); CyclinA2: 2-432(end)
Specific Activity
205 pmol/min/µg
Genbank #
CDK1: NM_001786; CyclinA2: NM_001237
UniProt #
CDK1: P06493; CyclinA2: P20248
Tag(s)
N-terminal GST-tag
Storage/Stability
At least 6 months at -80°C.
Assay Conditions
Assay was done according to the CDK1 Assay Kit (BPS Bioscience #79597) with various amounts of CDK1/CyclinA2.
Instructions for Use
Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
Applications
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Powered by Bioz
