CD40 - HEK293 Cell Line

Catalog #
71257
$4,400 *
Size: 2 vials
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

Recombinant HEK293 cell line expressing full length human CD40 (Tumor necrosis factor receptor superfamily member 5; TNFRSF5), Genbank Accession No. NP_001241.1. Expression is confirmed by real-time qPCR and Western blot.

This product has been cited 1 time.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

Product Info
Storage and Usage
Citations1
Host Cell Line
Human Embryonic Kidney cell line (HEK293). Adherent epithelial cells.
Format
Each vial contains ~2 x 10^6 cells in 1mL of 10% DMSO in FBS.
UniProt #
P29965
Mycoplasma Testing
This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza, Cat. #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, Cat. #LT07-518) was used as a positive control.
Background
CD40, a TNF receptor superfamily member, was initially identified on B lymphocytes. However, other cell types such as monocytes, basophils, dendritic cells, endothelial cells, and epithelial cells have been found to express CD40. High levels of CD40 have also been detected in numerous human cancers, including HCT116, a colon cancer epithelial cell line. Interaction with CD40 ligand (CD40L, CD154) on CD4+ T helper lymphocytes triggers the expression of intercellular adhesion molecule (ICAM) and other pro-inflammatory cytokines. CD40:CD40L signaling simultaneously increases activation of antigen-specific T cells. CD40 also activates NF-κB-dependent signaling in response to lipopolysaccharide (LPS) found on Gram negative bacterial pathogens. Agonistic CD40 monoclonal antibodies have been shown to activate antigen presenting cells (APC), promote anti-tumor T-cell responses, and to foster cytotoxic myeloid cells, suggesting a potential mechanism to control cancer in the absence of T-cell immunity.
References
1. Diaconu I. et.al. (2012) Immune Response is an Important Aspect of the Anti-tumor Effect Produced by a CD40L-Encoding Oncolytic Adenovirus. Cancer Res. 72: 2327.

2. Yacoub D et.al. (2013) CD154 is Released from T-cells by a Disintegrin and Metalloproteinase Domain-containing Protein 10 (ADAM10) and ADAM17 in a CD40 Protein-dependent Manner. J. Biol. Chem. 288: 36083.