CD40, Fc Fusion (IgG1) Avi-Tag, Biotin Labeled Recombinant

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Catalog #

79102

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Description

Human CD40, also known as Tumor Necrosis Factor Receptor Superfamily Member 5, TNFRSF5, Bp50. GenBank Accession No. NM_001250, a.a. 21-193, fused at the C-terminus with the Fc portion of Human IgG1, with C-terminal Avi-tag™, expressed in a HEK293 cell expression system. MW = 48 kDa. This protein runs at a higher molecular weight due to glycosylation.

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Synonyms

CD40, Tumor Necrosis Factor Receptor Superfamily Member 5, TNFRSF5, Bp50

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Western Blot

CD40: CD40L Interaction

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

HEK293

Purity

≥90%

Format

Aqueous buffer solution

Formulation

8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.

48 kDa

Endotoxin Level

<1 EU/µg

Amino Acids

21-193

Genbank #

NM_001250

UniProt #

P25942

Tag(s)

Fc (IgG1), Avi-Tag, Biotin Labeled

References

1. Lutgens, E. et al., Nat. Med. 1998; 5: 1313-1316.

2. Quezada, S.A., et al., Immunology. 2003; 22: 307-328

Storage/Stability

At least 6 months at -80°C

Assay Conditions

Reaction done according to standard ELISA assay kit protocol (e.g., Catalog # 72005). Buffer is PBS + 0.1% BSA. Blocking buffer is Superblock + 0.05% Tween-20. Plate was coated with 100 ng/well CD40L (#71191) at 4°C overnight. Various concentrations of biotinylated CD40 (0-1 µM) added for 2h at RT. Strep-HRP added for 1h at RT.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Shipping Temperature

-80°C

Notes

The aggregation state of this product has been confirmed to be <10%.

Warnings

Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only