CD39, His-Tag (Human) Recombinant

Catalog #

71284

$340 *

Size: 50 µg

Qty

Bulk/Custom

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Description

Recombinant human C39, also known as ENTPD1 (ectonucleoside triphosphate diphosphohydrolase 1), encompassing amino acids 38-478. This construct contains a C-terminal His-tag (10xHis). The recombinant protein was affinity purified.

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Synonyms

Ectonucleoside triphosphate diphosphohydrolase 1, NTPDase 1, Ecto-ATP diphosphohydrolase 1, Ecto-apyrase, Lymphoid cell activation antigen, ENTPD1

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

CD39 Activity

CD39 inhibition curve using a colorimetric assay (#79278). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Construct

CD39 (38-478-His)

Host Species/Expression System

HEK293

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

52 kDa + glycans

Amino Acids

38-478

Glycosylation

This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Specific Activity

≥3000 pmol/min/μg

Genbank #

NM_001776

UniProt #

P49961

Tag(s)

C-terminal His-tag

Background

CD39 contributes to immune regulation through the hydrolysis of ATP to AMP in the rate limiting step of extracellular adenosine production. CD39 is expressed on the surface of multiple cells of the immune system including T-cells, Natural killer cells, B-cells, dendritic cells and macrophages. In addition, high levels of CD39 expression have been associated with the tumor microenvironment (TME). Research aimed at reducing immunosuppression by CD39 activity has resulted in the study of a small molecule inhibitor, POM1, and multiple anti-CD39 antibodies selected for blocking enzymatic activity or decreasing CD39 expression.

References

1. Deaglio, S. et al., Journal of Environmental Medicine. 2007 Jun 204(6): 1257-1265.

2. Borsellino, G. et al., Blood. 2007 Aug; 110(4):1225-1232.

Storage/Stability

At least 6 months at –80°C

Assay Conditions

Assay was done according to CD39 Inhibitor Screening Assay Kit (BPS Bioscience #79278) with various amounts of CD39.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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Datasheets/Protocols

71284 Lot 220726

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CD39, His-Tag (Human) Recombinant

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