CD28H, Fc fusion, Biotin-labeled (Human) HiP™ Recombinant

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Catalog #

71239

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Description

Human CD28 homolog (CD28H), also known as Transmembrane and immunoglobulin domain-containing protein 2 (TMIGD2) and immunoglobulin and prolinerich receptor 1 (IGPR-1). GenBank Accession No. NM_144615, a.a. 23-128 fused at the C-terminus to the Fc portion of human IgG1 followed by a C-terminal AviTag™ expressed in a HEK293 expression system. MW = 40 kDa. This protein is enzymatically biotinylated using Avi-tag™ technology. Biotinylation is confirmed to be greater than 90%. This protein runs at a higher MW by SDS-PAGE due to glycosylation.

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Synonyms

CD28 homolog, Transmembrane and immunoglobulin domain-containing protein 2 TMIGD2, immunoglobulin and proline-rich receptor 1, IGPR-1

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Gel Filtration Curve

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

HEK293 cells

Purity

≥90%

Format

Aqueous buffer solution

Formulation

8 mM phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl, 20% glycerol.

40 kDa

Amino Acids

23-128

Genbank #

NM_144615

UniProt #

P10747

Tag(s)

C-terminal Fc fusion (Human IgG1), C-terminal Avi-tag™

References

1. Zhu, Y., et al., Nat. Commun. 2013; 4:2043.
2. Zhu, Y., et al., J. Immunol. 2015; 194:142.2.

Storage/Stability

At least 6 months at –80°C.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for studying protein binding and for screening small molecules and antibodies.

Shipping Temperature

-80°C

Notes

HiP™ indicates a high purity protein with ≥90% purity as measured by gel filtration.

Warnings

Avoid freeze/thaw cycles. Protein may be diluted to ≥100 μg/ml in PBS + glycerol and stored at -80°C.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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Datasheets/Protocols

71239 Lot 150901-G

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CD28H, Fc fusion, Biotin-labeled (Human) HiP™ Recombinant

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