CD2, Fc fusion, Avi-Tag, (Human) HiP™ Recombinant

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Catalog #

71264

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Description

Human secreted CD2-Fc fusion protein, also known as LFA-2, GenBank Accession No. NM_001767, a.a. 25-209, fused at the C-terminus to the Fc portion of human IgG1, with C-terminal Avi-tag, expressed in a HEK293 cell expression system. MW = 50 kDa (monomer). This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.

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Synonyms

LFA-2

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Gel Filtration Curve

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

HEK293

Purity

≥90%

Formulation

8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

50 kDa+glycans

Amino Acids

25-209

Genbank #

NM_001767

UniProt #

P06729

Tag(s)

C-terminal Avi-tag™

References

1. Seed, B., et al., Nature. 1987 Oct; 329: 840-842.

2. Seed, B., et al., PNAS. 1987 May; 84: 3365-3369.

Storage/Stability

At least 6 months at –80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of protein-protein interaction and in vitro and in vivo protein function studies.

Shipping Temperature

-80°C

Notes

HiP™ indicates a high purity protein with >90 percent purity by gel filtration. This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Warnings

Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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Datasheets/Protocols

71264.pdf

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CD2, Fc fusion, Avi-Tag, (Human) HiP™ Recombinant

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