CD155 (PVR), His-tag (Human) Recombinant

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Catalog #

71181

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Description

Human CD155, also known as Poliovirus receptor and Nectin-like protein 5, GenBank Accession No. NM_006505, a.a. 27-343 with a C-terminal His-tag, expressed in a HEK293 cell expression system. MW = 35 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.

This product has been cited 2 times.

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Synonyms

PVR, NECL-5, CD155-His

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Product Data Gallery

4-20% SDS-PAGE Coomassie staining

TIGIT: CD155 Interaction

CD226:CD155 inhibition curve using an AlphaLISA assay (#72052). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations2

Species

Human

Host Species/Expression System

HEK293

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.

35 kDa

Endotoxin Level

<1 EU/µg

Amino Acids

27-343

Genbank #

NM_006505

UniProt #

P15151

Tag(s)

C-terminal His-tag

References

1. Bottino, C. et al., J. Exp. Med. 2003; 198(4): 557-567.

2. Pende, D. et al., Blood. 2005; 105(5): 2066-2073

Storage/Stability

At least 6 months at –80°C.

Assay Conditions

CD155, His-tag binding to TIGIT was confirmed according to the protocol for BPS Cat. 72029. Briefly, 4 ng of TIGIT-biotin was mixed with varying amounts of CD155, His-tag in TIGIT assay buffer at a final volume of 10 µl and allowed to incubate for 1 hr at room temp. Nickle acceptor beads were added for 30 min, followed by Strep-conjugated acceptor beads for 30 min and reading alpha-counts

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Shipping Temperature

-80°C

Notes

The aggregation state of this product has been confirmed to be <10%.

Warnings

Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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Datasheets/Protocols

71181.pdf

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CD155 (PVR), His-tag (Human) Recombinant

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