CD137 (4-1BB) HEK293 Recombinant Cell Line

Catalog #: 60691
Size: 2 vials
$3,650
Species: Human
Genbank #: Genbank #NM_003811
Background:
Human CD137 (4-1BB; TNFRS9) is an inducible costimulatory molecule that activates T cells. CD137-CD137L mediated signaling has been shown to be important for proliferation, effector functions and survival of T cells. CD137 is also expressed in NK and NKT cells. Antibodies targeting CD137 activation in immune cells have demonstrated potent anti-tumor effects in cancer patients.
Description:
Recombinant HEK293 cell constitutively expressing full length human CD137 (Genbank #NM_003811). Surface expression is confirmed by flow cytometry.
Synonym(s):
4-1BB, TNFRS9, Mitogen-Activated Protein Kinase 14, Signal Transducer And Activator Of Transcription 3, STAT3, MAPK14
Supplied As:
Each vial contains ~ 2 x 106 cells in 1 ml of 10% DMSO in FBS.
Host Cell line:
Human Embryonic Kidney cell line (HEK293). Adherent epithelial cells.
Mycoplasma Testing:
This cell line has been screened using the MycoAlertâ„¢ Mycoplasma Detection Kit (Lonza, #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, #LT07-518) was used as a positive control.
Growth Media:

Thaw Medium 1 (BPS, #60187): MEM medium (Hyclone, #SH30024.01) supplemented with 10% FBS (Invitrogen, #26140-079), 1% non-essential amino acids (Hyclone, #SH30238.01), 1 mM Na pyruvate (Hyclone, #SH30239.01), 1% Penicillin/Streptomycin (Hyclone, #SV30010.01)

Growth Medium 1F (BPS, #79540): Thaw Medium 1 (BPS, #60187) plus 100 µg/ml Hygromycin B (Thermo Fisher, #10687010).

Instructions for use:
See data sheet for detailed culturing and assay protocol
Storage / Stability:
Immediately upon receipt, store vials in liquid nitrogen.
Application(s):
This cell line is useful for CD137 binding assays or downstream CD137-dependent signaling events.
Reference(s):

1. McNamara II, J.O., et.al. (2008) Multivalent 4-1BB binding aptamers co-stimulate CD8+ T cells and inhibit tumor growth in mice. J. Clin. Invest. 118: 376-386.

2. Ma, B.Y., et.al. (2005) The expression and regulatory role of OX40 and 4-1BB heterodimers in activated human T cells. Blood. 106: 2002-2010.

Notes:
License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit the use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Immunotherapy
Product Type:
Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

60691.pdf