CD112R (PVRIG), Fc-Fusion, Avi-Tag, Biotin-Labeled (Human) HiP™ Recombinant

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Catalog #

79270

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Description

Recombinant human CD112R, also known as PVRIG (Poliovirus receptor-related Immunoglobulin domain-containing protein), encompassing amino acids 41-172. This construct containsan C-terminal Human IgG1, Fc region followed by an Avi-Tag™. The recombinant protein was affinity purified. HiP™ indicates a high purity protein with ≥90% purity as measured by gel filtration.

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Synonyms

Poliovirus Receptor-related Immunoglobulin Domain-containing Protein, Transmembrane Protein PVRIG

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Anti-PVRIG:PVRIG Biotin Binding Assay. *Not all lots have been tested for enzyme activity

Biotin-Avidin Pulldown

Gel Filtration Curve

Product Info

Storage and Usage

Citations

Species

Human

Construct

CD112R (41-172-Fc-Avi)-(Biotin)

Host Species/Expression System

HEK293

Purity

≥90%

Format

Aqueous buffer solution

Formulation

8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.

42 kDa

Amino Acids

41-172

Aggregation

<10%

Genbank #

NM_024070

UniProt #

Q6DKI7

Tag(s)

C-terminal Fc-Fusion- Avi-Tag

Label

This protein is enzymatically biotinylated using Avi-Tag™ technology. Biotinylation confirmed to be ≥90%.

For more information on enzymatic biotinylation, please see our Tech Note.

Background

CD112R (Poliovirus receptor-related immunoglobulin domain containing, PVRIG) is the receptor for CD112 (Poliovirus receptor-related 2, PVRL2) found on antigen-presenting cells and tumor cells. CD112R is present in NK and T cells, particularly CD8+ T cells. Elevated levels of this receptor are observed in NK, CD8+, and CD4+ T cells in patients with cancer of the kidney, ovary, lung, prostate, and endometrium, and in acute myeloid leukemia (AML). CD112-CD112R complex acts as a positive immune checkpoint, boosting human T cell response and serving as a promising therapeutic target in oncology. TIGIT (T cell immunoreceptor with Ig and ITIM domains) and CD226 (also known as DNAM-1, DNAX Accessory Molecule-1) also bind CD112. Developing inhibitors to hinder CD112R-CD112 interaction alone or combined with blocking TIGIT-CD155/CD112 interaction holds promise for cancer therapy.

References

1. Bottino, C., et al., J. Exp. Med. 2003; 198(4): 557-567.

2. Pende, D., et al., Blood. 2005; 105(5): 2066-2073

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

The antibody was validated by measuring anti-PVRIG binding to PVRIG antigen in an ELISA assay. The anti-PVRIG protein (BPS Bioscience #101712) was coated onto a 96-well plate overnight at 4°C (50 µl/well at a concentration of 2 µg/ml in PBS). The plate was washed 3 times with Immuno Buffer 1 (BPS Bioscience #79311) and blocked using 100 µl of Blocking Buffer 2 (BPS Bioscience #79728) for 1 hour at room temperature. After removing the blocking buffer, 50 µl/well of serially diluted CD112R (PVRIG) Fc-Fusion, Avi-Tag, Biotin-Labeled (Human) (BPS Bioscience #79270), in Blocking Buffer 2 was added for 1 hour at room temperature. After 3 more washes, the plate was incubated with Streptavidin-HRP (BPS Bioscience #79742), washed, and incubated with the Colorimetric HRP substrate. The reaction was stopped, and absorbance was read at 450 nm. The Blank value was subtracted from all values.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for binding of PVRIG in ELISA and in cellular assays.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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Datasheets/Protocols

79270 Lot 170824

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CD112R (PVRIG), Fc-Fusion, Avi-Tag, Biotin-Labeled (Human) HiP™ Recombinant

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