Recombinant human CD112R, also known as PVRIG (Poliovirus receptor-related Immunoglobulin domain-containing protein), encompassing amino acids 41-172. This construct contains aC-terminal Human IgG1, Fc regionfollowed byan Avi-tag™. This recombinant protein was affinity purified.
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Synonyms
Poliovirus Receptor-related Immunoglobulin Domain-containing Protein, Transmembrane Protein PVRIG
8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
MW
42 kDa + glycans
Amino Acids
41-172
Glycosylation
This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Aggregation
<10%
Genbank #
NM_024070
UniProt #
Q6DKI7
Tag(s)
C-terminal Fc-Fusion- Avi-Tag
Background
PVRIG, also known as Poliovirus receptor related immunoglobulin domain containing protein or CD112R, is a natural killer (NK) cell immune checkpoint receptor, and it is also found in CD8+ T cells. PVRIG works as an inhibitory receptor and recognizes CD112 (also known as poliovirus receptor-related 2, PVRL2 or nectin-2)as its ligand. This binding results in lower responses of NK and T cells towards cancer cells. In T cells it inhibits the nuclear factor of activated T cells (NFAT). Blocking the interaction between PVRIG and its ligand, for instance with an antibody directed towards PVRIG, represents a novel strategy in checkpoint blockade immunotherapy.
References
1. Bottino, C., et al., J. Exp. Med. 2003; 198(4): 557-567.
2. Pende, D., et al., Blood. 2005; 105(5): 2066-2073
Storage/Stability
At least 6 months at –80°C.
Assay Conditions
Assay run according to CD112R:CD112[Biotinylated] Inhibitor Screening Assay Kit (BPS Bioscience #79732). 100 ng of CD112R was used to coat plates overnight at 4°C. Various amounts of CD112 [Biotinylated] were incubated for 2 hours at room temperature.
Instructions for Use
Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
Applications
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Shipping Temperature
-80°C
Notes
HiP™ indicates a high purity protein with ≥90% purity as measured by gel filtration.