Cathepsin V Inhibitor Screening Assay Kit
The Cathepsin V Inhibitor Screening Assay Kit is designed to measure the protease activity of Cathepsin V for screening and profiling applications. The Cathepsin V assay kit comes in a convenient 96-well or 384-well format, with enough purified Cathepsin V (amino acids 18-334), its substrate, and Cathepsin Buffer for 96 or 384 reactions. This kit includes the inhibitor E-64 as control.
Figure 1: Illustration of the assay principle.
The substrate is an internally quenched fluorogenic substrate. Proteolysis releases the highly fluorescent Mca from the quencher. Fluorescence intensity increases proportionally to the activity of the protease
Need us to run inhibitor screens or profile your compounds against Cathepsin V? Check out our Protease Screening Services.
- Adjustable micropipettor and sterile tips
- Fluorescence plate reader capable of measurement at λex330/λem390 nm.
96 reactions
| Catalog # | Name | Amount | Storage |
| 80009 | Cathepsin V, His-Tag* | ˃1 µg | -80°C |
| 80349 | Fluorogenic Cathepsin Substrate 1 (5 mM) | 10 µl | -20°C |
| 4x Cathepsin Buffer | 2 ml | -20°C | |
| 0.5 M DTT | 200 µl | -20°C | |
| 1 mM E-64 | 10 µl | -20°C | |
| 79685 | 96-well black microplate | 1 | Room Temp |
* The concentration of protein is lot-specific and will be indicated on the tube containing the protein.
384 reactions
| Catalog # | Name | Amount | Storage |
| 80009 | Cathepsin V, His-Tag* | ˃1 µg | -80°C |
| 80349 | Fluorogenic Cathepsin Substrate 1 (5 mM) | 2 x 10 µl | -20°C |
| 4x Cathepsin Buffer | 2 x 2 ml | -20°C | |
| 0.5 M DTT | 2 x 200 µl | -20°C | |
| 1 mM E-64 | 10 µl | -20°C | |
| 79961 | 384-well black microplate | 1 | Room Temp |
* The concentration of protein is lot-specific and will be indicated on the tube containing the protein.
Cathepsin V, also called Cathepsin L2, is a lysosomal cysteine endopeptidase with high sequence homology to cathepsin L and other members of the papain superfamily of cysteine proteinases. Its expression is regulated in a tissue-specific manner and is high in thymus, heart, brain, testis, and cornea. It is involved in maturation of MHC class II molecules, turnover of elastin and breakdown of intra and extra-cellular substrates. Expression analysis of cathepsin V in human tumors revealed widespread expression in colorectal and breast carcinomas, suggesting a possible role in tumor processes. It has also been linked to atherosclerosis, hypertension, and aortic aneurysm, amongst other vascular-related pathologies. Further studies into its functions will allow us to understand in depth the potential benefits of targeting Cathepsin V for therapeutic purposes.
Lecaille F., et al., 2022 Molecular Aspects of Medicine 88: 101086.
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