Cathepsin V, His-tag Recombinant

Catalog #

80009

$385 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human cathepsin V, encompassing amino acids 18-334(end). This construct has a C-terminal His-tag (10xHis). This protein was affinity purified.

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Synonyms

CTSV

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Cathepsin V Activity

Cathepsin V inhibition curve using a fluorogenic assay (#79589). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations

Species

Human

Construct

Cathepsin V (18-334(end)-His)

Host Species/Expression System

HEK293

Purity

≥79%

Format

Aqueous buffer solution

Formulation

40 mM MES, pH 5.0, 120 mM NaCl, and 20% glycerol.

37 kDa + glycans

Amino Acids

CD33 1–16, CathepsinV 18–334

Glycosylation

This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Specific Activity

≥ 250 pmol/min/µg

Genbank #

NM_060911

UniProt #

O60911

Tag(s)

C-terminal His-tag

Background

Cathepsin V, also called Cathepsin L2, is a lysosomal cysteine endopeptidase with high sequence homology to cathepsin L and other members of the papain superfamily of cysteine proteinases. Its expression is regulated in a tissue-specific manner and is high in thymus, heart, brain, testis, and cornea. It is involved in maturation of MHC class II molecules, turnover of elastin and breakdown of intra and extra-cellular substrates. Expression analysis of cathepsin V in human tumors revealed widespread expression in colorectal and breast carcinomas, suggesting a possible role in tumor processes. It has also been linked to atherosclerosis, hypertension, and aortic aneurysm, amongst other vascular-related pathologies. Further studies into its functions will allow us to understand in depth the potential benefits of targeting Cathepsin V for therapeutic purposes.

Storage/Stability

>6 months at -80°C.

Assay Conditions

Assay was done according to Cathepsin V Inhibitor Screening Assay Kit (BPS Bioscience #79589) with various amounts of Cathepsin V, His-Tag Recombinant.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Protease

Regulatory Status

Research Use Only