Caspase3, His-tag

Catalog #: 80500
Size: 50 µg
$425
Species: Human
Host Species: E. coli
MW: see Description
Genbank #: NM_004346
Tag(s): C-terminal His-tag
a.a: 1 - 277 (end)
Background:
Caspase-3 is a member of the caspase (cysteine aspartate protease) family of proteins, and has been shown to be an executioner protein of apoptosis.
Description:
Human caspase 3 (CASP3), also known as CPP32 and apopain, a.a. 1 – 277 (end) (GenBank Accession No. NM_004346, var alpha) with C-terminal His-tag, expressed in an E. coli expression system. Procaspase-3 is self-cleaved into two subunits, MW = 17 kDa and 13 kDa, to form the active heterotetrameric complex.
UniProt
P42574
Synonym(s):
caspase-3, CASP3, caspase 3, CPP32, apopain, Yama
Specific Activity:
≥ 6,784 pmol/min/µg
Assay Conditions:
Assayed using BPS's Homogeneous Caspase-3 Assay Kit, Cat. # 80700. Assay conditions: 10 mM HEPES, pH 7.4, 10 mM EDTA, 0.05% Chaps, 5 mM DTT, and 20 µM Caspase 3 substrate. Incubate for 30 minutes at room temperature. Fluorescence intensity is measured at exc380/em500 nm.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol and 3 mM DTT.
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors,and selectivity profiling.
Reference(s):
1. Lee, D., et al., J Biol Chem. 2000 May 26;275(21):16007-14
2. Suzuki, Y., et al., Proc Natl Acad Sci USA. 2001 Jul 17;98(15):866-7.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Protease
Product Type:
Recombinant Protein
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80500_Lot130226-G.pdf