Caspase3, His-Tag Recombinant

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Catalog #

80500

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Description

Recombinant human Caspase3 (CASP3), full length, encompassing amino acids 1-277(end). This construct contains a C-terminal His tag (6xHis). Procaspase-3 is self-cleaved into two subunits, MW = 17 kDa and 13 kDa, to form the active heterotetrameric complex. The recombinant protein was affinity purified.

This product has been cited 1 time.

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Synonyms

caspase-3, CASP3, caspase 3, CPP32, apopain, Yama, Caspase

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Caspase3 Activity

Caspase-3 inhibition curve using a fluorogenic assay (#80700). Representative data only; data may vary by lot.

Product Info

Storage and Usage

Citations1

Species

Human

Construct

Caspase3 (1-277(end)-His)

Host Species/Expression System

E. coli

Purity

≥90%

Format

Aqueous buffer solution

Formulation

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 3 mM DTT

Small active subunit: 13 kDa; Large active subunit: 17 kDa

Amino Acids

1 - 277 (end)

Specific Activity

≥6784 pmol/min/µg

Genbank #

NM_004346

UniProt #

P42574

Tag(s)

C-terminal His-tag

Background

Caspase-3 is a member of the caspase (cysteine aspartate protease) family of proteins, and has been shown to be an executioner protein of apoptosis.

References

1. Lee, D., et al., J Biol Chem. 2000 May 26;275(21):16007-14
2. Suzuki, Y., et al., Proc Natl Acad Sci USA. 2001 Jul 17;98(15):866-7.

Storage/Stability

At least 6 months at -80°C.

Assay Conditions

Assay was done according to Caspase-3 Homogeneous Assay Kit (BPS Bioscience #80700) with various amounts of Caspase-3.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles.

Scientific Category

Protease

Regulatory Status

Research Use Only