CD38 CHO Recombinant Cell Line (High, Medium or Low Expression)

Catalog #: 79615-H
Size: 2 Vials
$6,000
Catalog #: 79615-M
Size: 2 Vials
$6,000
Catalog #: 79615-L
Size: 2 Vials
$6,000
Background:
CD38 (cyclic ADP-ribose hydrolase 1, ADPRC1) is a glycoprotein and ectoenzyme which plays an important role in regulating intracellular calcium. CD38 is a highly attractive target antigen for immunotherapy because it is highly expressed on multiple myeloma cells, and at relatively low levels on normal lymphoid and myeloid cells. Expression of CD38 has also been associated with HIV infection, leukemia, and type II diabetes mellitus. CD38 CAR-T is a T cell product engineered by retroviral transduction to express a fully human CD38-specific CAR (chimeric antigen receptor). In 2015, the FDA approved daratumumab (Darzalex), a breakthrough therapy drug targeting CD38, for the treatment of multiple myeloma.
Description:
Recombinant clonal stable CHO cell line constitutively expressing full length human CD38 protein, also known as ADPRC1 (Genbank #NM_001775). Surface expression of CD38 was confirmed by flow cytometry. Each stable clonal cell line was selected for different levels of CD38 expression (High, Medium, Low) to mimic different stages of cancer target cells with various CD38 expression levels.
Synonym(s):
cluster of differentiation 38, cyclic ADP ribose hydrolase
Supplied As:
Each vial contains ~ 2 x 10^6 cells in 1 ml of 10% DMSO in FBS.
Host Cell line:
CHO K1 cell line, Chinese Hamster Ovary
Mycoplasma Testing:
This cell line has been screened using the MycoAlertâ„¢ Mycoplasma Detection Kit (Lonza, #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, #LT07-518) was used as a positive control.
Growth Media:

Thaw Medium 3 (BPS Bioscience, #60186):  Ham’s F-12 medium (Hyclone #SH30526.01) supplemented with 10% FBS (Life technologies #26140-079), 1% Penicillin/Streptomycin (Hyclone #SV30010.01).   

Growth Medium 3D (BPS Bioscience, #79539): Thaw Medium 3 (BPS Bioscience, #60186) plus 1 mg/ml of G418 (Thermo Fisher, #11811031).

Instructions for use:
See data sheet for detailed culturing and assay protocol.
Storage / Stability:
Store in liquid nitrogen immediately upon receipt.
Application(s):
Useful as CD38-expressing target cells in co-culture assays with CD38 CAR-T cells, for both CD38-specific cell killing assay and cytokine production assay.
Notes:

LICENSE DISCLOSURE: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit the use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego. 

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
CAR T
Product Type:
Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.

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$6,000

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Product Datasheets

79615-H.pdf 79615-M.pdf 79615-L.pdf