CD20 CHO Recombinant Cell Line (High or Medium Expression)

Catalog #: 79624-H
Size: 2 vials
$6,000
Catalog #: 79624-M
Size: 2 vials
$6,000
Background:

CD20 (MS4A1) is a glycosylated phosphoprotein expressed on the cell surface of B cells. Although the functional significance of CD20 is not clear, and CD20 has no known ligands, CD20 has been shown to regulate intracellular calcium levels. CD20 is a highly attractive target antigen for immunotherapy because it is expressed on more than 90% of patients with B-cell lymphoma. First approved in 1997, Rituximab (Rituxan) is a chimeric monoclonal antibody targeting CD20 and has been classified by the World Health Organization as an “Essential Medicine”.

Since then, additional monoclonal antibodies against CD20 have been approved or are being tested in clinical trials for the treatment of multiple sclerosis (MS), chronic lymphocytic leukemia (CLL), follicular lymphoma, diffuse large B cell lymphoma (DLBCL), rheumatoid arthritis, non-Hodgkin’s lymphoma, systemic lupus erythematosus, and myalgic encephalomyelitis (chronic fatigue syndrome). Additionally, more recently, anti-CD20-CD19 bispecific CAR-T cells have been developed to address concerns over potential relapse.

Description:
Recombinant clonal stable CHO cell line constitutively expressing full length human CD20 protein, also known as MS4A1 (Genbank #NM_152866). Surface expression of CD20 was confirmed by flow cytometry. Each stable clonal cell line was selected for different levels of CD20 expression (High, Medium) to mimic different stages of cancer target cells with various CD20 expression levels.
Synonym(s):
rituximab
Supplied As:
Each vial contains ~ 2 x 10^6 cells in 1 ml of 10% DMSO in FBS.
Host Cell line:
CHO K1 cell line, Chinese Hamster Ovary
Mycoplasma Testing:
This cell line has been screened using the MycoAlertâ„¢ Mycoplasma Detection Kit (Lonza, #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, #LT07-518) was used as a positive control.
Growth Media:

Thaw Medium 3 (BPS Bioscience, #60186):  Ham’s F-12 medium (Hyclone #SH30526.01) supplemented with 10% FBS (Life technologies #26140-079), 1% Penicillin/Streptomycin (Hyclone #SV30010.01).   

Growth Medium 3D (BPS Bioscience, #79539): Thaw Medium 3 (BPS Bioscience, #60186) plus 1 mg/ml of G418 (Thermo Fisher, #11811031).

Instructions for use:
See data sheet for detailed culturing and assay protocol.
Storage / Stability:
Store in liquid nitrogen immediately upon receipt.
Application(s):
Useful as CD20-expressing target cells in co-culture assays with CD20-CAR-T cells for CD20-specific cell killing assays.
Notes:

LICENSE DISCLOSURE: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit the use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego. 

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
CAR T
Product Type:
Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.

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$6,000

Quality Assurance

Product Datasheets

79624-H.pdf 79624-M.pdf