CD38 Inhibitor Screening Assay Kit (Hydrolase Activity)

Catalog #
79287
$890 *
Size: 96 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

The CD38 Inhibitor Screening Assay Kit (Hydrolase Activity) is designed to measure the glycohydrolase activity of CD38 for screening and profiling applications. The CD38 assay kit comes in a convenient 96-well format, with purified recombinant CD38 enzyme, its substrate N6-etheno-NAD (ε-NAD), and CD38 assay buffer for 100 enzyme reactions. In addition, the kit includes the CD38 inhibitor apigenin for use as a control inhibitor.

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Synonyms
cluster of differentiation 38, cyclic ADP ribose hydrolase, activity assay kit
Product Info
Storage and Usage
Citations
Assay Kit Format
Fluorogenic
Materials Required But Not Supplied

Adjustable micropipettor and sterile tips
Fluorescent microplate reader
Rotating or rocker platform

Format
Catalog
Number
Reagent Amount Storage
71277 CD38, His-Tag (Human), HiP™ 1 µg -80°C

Avoid
mulitple
freeze/thaw
cycles!

4x CD38 hydrolase buffer 3 ml -20°C
CD38 substrate (ε-NAD) 50 µl -20°C
Apigenin (50 mM DMSO) 10 µl -20°C
79685 Black 96-well plate 1 Room Temp.
UniProt #
P28907
Background
CD38, a differentiation antigen of B lymphocytes, is a type II integral membrane protein. It is also known as ADP-ribosyl cyclase and nicotinamide adenine dinucleotide (NAD) glycohydrolase. Through its production of cyclic ADP-ribose, CD38 modulates calcium-mediated signal transduction in various cells, including pancreatic β cells. The major enzymatic activity of CD38 is the hydrolysis of NAD. CD38 is a prognostic biomarker for acute B lymphoblastic leukemia.
References

Wei, W., et al., World J. Biol. Chem. 2014 5(1):58-67