SLAMF7 (CS1) CHO Recombinant Cell Line (High, Medium, or Low Expression)

Catalog #: 79608-H
Size: 2 Vials
$6,000
Catalog #: 79608-M
Size: 2 Vials
$6,000
Catalog #: 79608-L
Size: 2 Vials
$6,000
Background:
The surface antigen SLAMF7 is expressed on a fraction of normal lymphocytes, including subsets of natural killer (NK) cells, T cells, and B cells. It is a robust marker of normal plasma cells and malignant plasma cells in multiple myeloma. In contrast to CD138 (the traditional plasma cell marker), CD319/SLAMF7 is much more stable and allows robust isolation of malignant plasma cells from patient samples. SLAMF7 is under intense investigation as a target for immunotherapy in multiple myeloma. It has been demonstrated that SLAMF7-CAR T cells prepared from patients and healthy donors confer potent antimyeloma reactivity. SLAMF7-CAR T cells confer fratricide of SLAMF7+/high normal lymphocytes. SLAMF7-CAR T cells represent a novel therapeutic agent for the treatment of patients with SLAMF7-expressing multiple myeloma malignancies.
Description:
Recombinant clonal stable CHO cell line constitutively expressing full length human SLAMF7 protein, also known as CS1 or CD319 (Genbank #NM_021181). Surface expression of SLAMF7 was confirmed by flow cytometry. Each stable clonal cell line was selected for different levels of SLAMF7 expression (High, Medium, Low) to mimic different stages of cancer target cells with various SLAMF7 expression levels.
Synonym(s):
CS1, CD319
Supplied As:
Each vial contains ~ 2 x 10^6 cells in 1 ml of 10% DMSO in FBS.
Host Cell line:
CHO K1 cell line, Chinese Hamster Ovary
Mycoplasma Testing:
This cell line has been screened using the MycoAlertâ„¢ Mycoplasma Detection Kit (Lonza, #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, #LT07-518) was used as a positive control.
Growth Media:

Thaw Medium 3 (BPS Bioscience, #60186):  Ham’s F-12 medium (Hyclone #SH30526.01) supplemented with 10% FBS (Life technologies #26140-079), 1% Penicillin/Streptomycin (Hyclone #SV30010.01).   

Growth Medium 3D (BPS Bioscience, #79539): Thaw Medium 3 (BPS Bioscience, #60186) plus 1 mg/ml G418 (Thermo Fisher, #11811031).

Instructions for use:
See data sheet for detailed culturing and assay protocol.
Storage / Stability:
Store in liquid nitrogen immediately upon receipt.
Application(s):
Useful as SLAMF7-expressing target cells in co-culture assay with SLAMF7-CAR T cells, for both SLAMF7-specific cell killing assay and cytokine production assay.
Reference(s):

1. Kramer, B., et al. Role of the NK cell-activating receptor CRACC in periodontitis. Infect. Immun. 81(3): 690-696 (2013) 

2. Boles, K.S., et al. Molecular cloning of CS1, a novel human natural killer cell receptor belonging to the CD2 subset of the immunoglobulin superfamily. Immunogenetics  52(3-4): 302-307 (2001)

3. Gogishvili, T., et al. SLAMF7-CAR T cells eliminate myeloma and confer selective fratricide of SLAMF7+ normal lymphocytes.  Blood. 130: 2838-2847 (2017) 

Notes:

LICENSE DISCLOSURE: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit the use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego. 

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Immunotherapy
Product Type:
Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.

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$6,000

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Product Datasheets

79608-H.pdf 79608-M.pdf 79608-L.pdf