HER2 (ERBB2) CHO Recombinant Cell Line (High, Medium, or Low Expression)

Catalog #: 79612-M
Size: 2 vials
$6,000
Catalog #: 79612-L
Size: 2 vials
$6,000
Catalog #: 79612-H
Size: 2 vials
$6,000
Background:
Receptor tyrosine-protein kinase erbB-2 (HER2 or CD340) is a protein that in humans is encoded by the ERBB2 gene. Over-expression of this oncogene has been shown to play an important role in the development and progression of certain aggressive types of breast cancer. HER2 over-expression is also known to occur in ovarian, stomach, adenocarcinoma of the lung, aggressive forms of uterine cancer and gastric cancer. Signaling through the ErbB family of receptors promotes cell proliferation and opposes apoptosis, and therefore must be tightly regulated to prevent uncontrolled cell growth from occurring.
Description:
Recombinant clonal stable CHO cell line constitutively expressing full length human ERBB2 protein (Genbank NM_004448.2). Surface expression of ERBB2 was confirmed by flow cytometry. Each stable clonal cell line was selected for different levels of ERBB2 expression (High, Medium, Low) to mimic different stages of cancer target cells with various ERBB2 expression levels.
Synonym(s):
HER2, NEU, ErbB2, TKR1, CD340, human epidermal growth factor receptor 2
Supplied As:
Each vial contains ~ 2 x 10^6 cells in 1 ml of 10% DMSO in FBS.
Host Cell line:
CHO K1 cell line, Chinese Hamster Ovary
Mycoplasma Testing:
This cell line has been screened using the MycoAlertâ„¢ Mycoplasma Detection Kit (Lonza, #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, #LT07-518) was used as a positive control.
Growth Media:

Thaw Medium 3 (BPS Bioscience, #60186):  Ham’s F-12 medium (Hyclone #SH30526.01) supplemented with 10% FBS (Life technologies #26140-079), 1% Penicillin/Streptomycin (Hyclone #SV30010.01).   

Growth Medium 3B (BPS Bioscience, #79529): Thaw Medium 3 (BPS Bioscience, #60186) plus 500 µg/ml Hygromycin (Thermo Fisher, #10687010).

Instructions for use:
See data sheet for detailed culturing and assay protocol.
Storage / Stability:
Store in liquid nitrogen immediately upon receipt.
Application(s):
Screen for activators or inhibitors of ERBB2 antibody-mediated cell signaling for immunotherapy research and drug discovery.
Notes:

LICENSE DISCLOSURE: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit the use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego. 

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
CAR T
Product Type:
Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.

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$6,000

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Product Datasheets

79612-H.pdf 79612-M.pdf 79612-L.pdf