Firefly Luciferase - CHO Recombinant Cell Line

Catalog #

79725

$1,895 *

Size: 2 Vials

Qty

Bulk/Custom

Add to Favorites

*US Pricing only. For international pricing, please contact your local distributor.

●

Purchase

●

Description

Recombinant CHO-K1 cells constitutively expressing the firefly luciferase reporter.

This product has been cited 4 times.

Purchase of this cell line is for research purposes only; commercial use requires a separate license. View the full terms and conditions.

●

Product Data Gallery

Luciferase activity of Firefly Luciferase CHO recombinant cells compared with BCMA / Luciferase CHO recombinant cells

Product Info

Storage and Usage

Citations4

Host Cell Line

Chinese Hamster Ovary (CHO)-K1, adherent epithelial cells.

Supplied As

Each vial contains ~ 2 x 106 cells in 1 ml of 10% DMSO in FBS

Materials Required But Not Supplied

• Thaw Medium 3 (BPS Bioscience, #60186)
• Growth Medium 3D (BPS Bioscience, #79539)
• 96-well tissue culture-treated white clear-bottom assay plate
• ONE-Step luciferase assay system (BPS Bioscience, #60690) or other luciferase reagent for measuring firefly luciferase activity
• Luminometer

Mycoplasma Testing

This cell line has been screened using the Venor™ GeM Mycoplasma Detection Kit, PCR Based (Sigma, #MP0025) to confirm the absence of Mycoplasma contamination.

Background

Since CHO-K1 cells do not express endogenous human proteins, this cell line provides an excellent platform to compare specific killing of CAR-T or NK cells relative to CHO-K1 cells expressing both the firefly luciferase and a specific cell surface receptor of interest, such as B-Cell Maturation Antigen (BCMA) (BPS Bioscience #79724).

Storage/Stability

Store in liquid nitrogen immediately upon receipt.

Growth Media

For best results, it is highly recommended to use these validated and optimized media from BPS Bioscience. Other preparations or formulations of media may result in suboptimal performance.

Thaw Medium 3 (BPS Bioscience, #60186): F-12K Medium supplemented with 10% FBS and 1% Penicillin/Streptomycin

Growth Medium 3D (BPS Bioscience, #79539): F-12K Medium supplemented with 10% FBS and 1% Penicillin/Streptomycin plus 1 mg/ml of Geneticin to ensure recombinant expression.

Shipping Temperature

-80°C

Notes

License Disclosure
Visit bpsbioscience.com/license for the label license and other key information about this product.

Troubleshooting Guide
Visit bpsbioscience.com/cell-line-faq for detailed troubleshooting instructions. For all further questions, please email [email protected].

Warnings

Avoid multiple freeze/thaw cycles.

Scientific Category

Cell Signaling Pathway

Regulatory Status

Research Use Only

Skip to the end of the images gallery

Skip to the beginning of the images gallery

Firefly Luciferase - CHO Recombinant Cell Line

PRODUCTS

SERVICES

RESOURCES

COMPANY