CAMKK1, GST-tag Recombinant

Catalog #

40044

$410 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Recombinant human CAMKK1, with N-terminal GST-tag, expressed by Sf9 insect cells via a baculovirus expression system.

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Synonyms

CAMKKA, MGC34095, DKFZp761M0423, CAMK

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥75%

Format

Aqueous buffer solution

Formulation

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.

94 kDa

Amino Acids

full length

Genbank #

NM_032294

UniProt #

Q8N5S9

Tag(s)

N-terminal GST-tag

Background

CAMKK1 or CAMKKα is a Ca(2+)/calmodulin-dependent protein kinase that activates CaM-kinases I and IV via phosphorylation of their Thr(177) and Thr(196) residues, respectively. Recent studies have shown that the activity of CAMKK1 is decreased upon phosphorylation by cAMP-dependent protein kinase (PKA) (1) The CAMKKalpha has been identified in intact cells as AMPKKs, predicting a significant role for this kinase in regulating AMPK activity in vivo. It has been shown that 2-deoxyglucose- and ionomycin-stimulated AMPK activity is substantially reduced in HeLa cells transfected with small interfering RNAs specific for CAMKKα (2).

References

1. Okuno, S. et al: Regulation of Ca(2+)/calmodulin-dependent protein kinase kinase alpha by cAMP-dependent protein kinase: I. Biochemical analysis. J Biochem (Tokyo). 2001 Oct;130(4):503-13.
2. Hurley, R L. et al: The Ca2+/calmodulin-dependent protein kinase kinases are AMP-activated protein kinase kinases. J Biol Chem. 2005 Aug 12;280(32):29060-6.

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CAMKK1, GST-tag Recombinant

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