CAMK2α, GST-tag Recombinant

Catalog #
40038
$410 *
Size: 10 µg
Qty
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Description

Recombinant human calcium/calmodulin-dependent protein kinase II alpha (CaMK2α), GenBank Accession #NM_171825, full length, with an N-terminal GST-tag, MW = 74 kDa, expressed in Sf9 cells via a baculovirus expression system.

Synonyms
CAMKA; KIAA0968, CAMK-2
Product Info
Storage and Usage
Citations
Species
Human
Host Species/Expression System
Sf9 insect cells
Purity

≥80%

Format
Aqueous buffer solution
Formulation
50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
MW
74 kDa
Amino Acids
full length
Specific Activity
294 pmol/min/µg
Genbank #
NM_171825
UniProt #
Q9UQM7
Tag(s)
N-terminal GST-tag
Background
CAMK2α is a ser/thr protein kinase that is a member of the Ca2+/calmodulin-dependent protein kinase family. CAMK2α is abundant in the brain as a major constituent of the postsynaptic density and is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its Ca2+/calmodulin-dependent activity, CAMK2α can undergo autophosphorylation, resulting in Ca2+/calmodulin-independent activity. The protein level of CAMK2α fluctuates during neuronal activity in cultured rat pup hippocampal neurons. The levels of CAMK2α increased with heightened neuronal activity (2).
References
1. Silva, A J. et a . Science 257: 206-211, 1992.
2. Thiagarajan, T C. et al . Neuron 36: 1103-1114, 2002.
3. Salas, M.A., et al. J. Mol. Cell Cardiol. (2010) 48(6):1298-130.
4. Chen, Y., et al. Neurochem Res. (2008) 33(10):2092-8.