CAMK1, Mouse, GST-tag

Catalog #: 40030
Size: 10 µg
$370
Species: Mouse
Host Species: E. coli
MW: 70 kDa
Genbank #: NM_133926
Tag(s): N-terminal GST-tag
a.a: full length
Background:
CAMK1 is a serine/threonine protein kinase that is a member of the multifunctional calcium/calmodulin-dependent protein kinase family. CAMK1 is ubiquitously expressed and phosphorylates a number of proteins including SYN1, SYN2, CREB and CFTR. In addition, Numb family of proteins may also be intracellular targets for CAMK1, and they may also be regulated by phosphorylation-dependent interaction with 14-3-3 protein (1). CAMK1 also plays an important role in the trafficking of HDAC7 between the cytoplasm and the nucleus. CAMK1 phosphorylates HDAC7 on multiple sites that lead to alteration in localization of HDAC7 (2).
Description:
Recombinant mouse CAMK1, full length, with N-terminal GST-tag, expressed in E. coli cells
Synonym(s):
CaMKIalpha, AI505105; D6Ertd263e
Purity:
≥75%
Formulation:
50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Tokumitsu, H. et al: Phosphorylation of Numb family proteins. Possible involvement of Ca2+/calmodulin-dependent protein kinases. J Biol Chem. 2005 Oct 21;280(42):35108-18.
2. Gao, C. et al: CRM1 mediates nuclear export of HDAC7 independently of HDAC7 phosphorylation and association with 14-3-3s. FEBS Lett. 2006 Sep 18;580(21):5096-104.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Kinase/Serine-Threonine
Product Type:
Recombinant Protein
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