BTLA, Fc Fusion, Avi-Tag, Biotin-Labeled HiP™ Recombinant

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Catalog #

100047

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Description

Human B- and T-lymphocyte attenuator BTLA, also known as CD272, GenBank Accession No. NM_181780, a.a. 31-150, with C-terminal Avi-Tag fused to the Fc region of Human IgG1, expressed in a HEK293 cell expression system. MW = 42 kDa (monomer). This protein is enzymatically biotinylated using Avi-TagTM technology.

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Synonyms

CD272

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

BTLA: HVEM Interaction

Gel Filtration Curve

Product Info

Storage and Usage

Citations

Species

Human

Purity

≥90%

Format

Aqueous buffer solution

Formulation

8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol

42 kDa

Amino Acids

31-150

Genbank #

No. NM_181780

UniProt #

Q7Z6A9

Tag(s)

C-terminal Fc-Avi-Tag

References

1. Flynn R, et al., PLoS One. 2013 Oct 29;8(10):77991.

2. Uchiyama M, et al., Transplantaion. 2014 Feb 15;97(3):301-9.

Storage/Stability

At least 6 months at –80°C

Assay Conditions

BTLA: HVEM interaction was tested using the BTLA: HVEM [Biotinylated] Inhibitor Screening Assay Kit (BPS Cat. #72008).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Shipping Temperature

-80°C

Notes

HiP™ indicates a high purity protein with ≥90% purity as measured by gel filtration.

Warnings

Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.

Scientific Category

Immunotherapy

Regulatory Status

Research Use Only

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Datasheets/Protocols

100047_Lot_180426-G.pdf

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BTLA, Fc Fusion, Avi-Tag, Biotin-Labeled HiP™ Recombinant

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