BTK Assay Kit

Catalog #

79568

$560 *

Size: 96 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

Bruton’s tyrosine kinase or BTK, is an enzyme that plays a role in the functionality and maturation of B cells. The BTK pathway has implications for a number of autoimmune disorders including isolated growth hormone deficiency type III and rheumatoid arthritis. The BTK Assay Kit is designed to measure BTK activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.

Need us to run inhibitor screens or profile your compounds against BTK? Check out our Kinase Screening Services.

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Product Data Gallery

Product Info

Storage and Usage

Citations

Assay Kit Format

Luminescent

Supplied As

The BTK Assay Kit comes in a convenient 96-well format, with enough purified recombinant BTK enzyme, PolyGluTyr peptide, ATP, and kinase assay buffer for 100 enzyme reactions.

Materials Required But Not Supplied

Kinase-Glo MAX (Promega #V6071)
Dithiothreitol (DTT, 1 M; optional)
Microplate reader capable of reading luminescence
Adjustable micropipettor and sterile tips
30°C incubator

Format

Catalog Number	Reagent	Amount	Storage
40405	BTK	20 µg	-80°C	Avoild multiple freeze/thaw cycles!
79334	5x Kinase assay buffer	1.5 ml	-20°C
79686	ATP (500 µM)	100 µl	-20°C
40217	Poly-(Glu,Tyr 4:1) (10 mg/ml)	100 µl	-20°C
79696	96-well plate, white	1	Room Temp.

UniProt #

Q06187

References

Rawlings, D., Saffran, D., Tsukada, S., Largaespada, D., Grimaldi, J., Cohen, L., et al. (1993). Mutation of unique region of Bruton’s tyrosine kinase in immunodeficient XID mice. Science 261(5119): 358–361.