BRSK2, His-tag Recombinant

Catalog #

40016

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human recombinant BRSK2 (GenBank Accession No. NM_003957), full length with N-terminal His-tag, MW=75 kDa, expressed in expressed in a baculovirus expression system.

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Synonyms

BRSK2, BR Serine/Threonine Kinase 2, SADA, STK29, SAD1, Brain-Selective Kinase 2

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Product Data Gallery

BRSK2 Specific Activity

10% SDS-PAGE Coomassie Staining

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Format

Aqueous buffer solution

Formulation

25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, and 10% glycerol.

75 kDa

Amino Acids

full length

Specific Activity

412 pmoles/min/µg

Genbank #

NM_003957

UniProt #

Q8IWQ3

Tag(s)

N-terminal His-tag

References

1. Bright N.J. et al., J. Biol. Chem. 283 (22), 14946-14954 (2008).
2. Guo Z. et al., Biochem. Biophys. Res. Commun. 347 (4), 867-871 (2006).

Storage/Stability

>6 months at -80°C.

Assay Conditions

Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, and 2 µM substrate (Ser/Thr 21, Invitrogen) at room temperature for 1 hour.

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Serine-Threonine

Regulatory Status

Research Use Only

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BRSK2, His-tag Recombinant

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