BRD4 (BD1), His-tag

Catalog #: 31042
Size: 100 µg
$295
Species: Human
Host Species: E. coli
MW: 15.5 kDa
Genbank #: NM_058243
Tag(s): N-terminal His-tag
a.a: 49-170
Description:
Human Bromodomain containing 4, also known as BRD4, HUNK1, or MCAP, GenBank Accession No. NM_058243, a.a. 49-170 corresponding to bromodomain 1 with N-terminal His-tag, MW = 15.5 kDa, expressed in an E. coli expression system.
UniProt
O60885
Synonym(s):
Bromodomain containing 4, BRD4, HUNK1, MCAP
Assay Conditions:

BRD4 is incubated with biotinylated acetyl-histone peptide substrate (BPS# 33000) in BRD assay buffer (BPS# 33001) in a 10 µl reaction for 1 hour at RT. AlphaLISA® ® Nickel Chelate Acceptor Beads (PerkinElmer) are added, followed by AlphaScreen® Streptavidin-conjugated donor beads (PerkinElmer). After 1 hour incubation at RT, Alphacounts are measured.

Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 1.28 µM imidazole, and 20% glycerol
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at –80°C.
Application(s):
Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
Reference(s):
1. Rodriguez, RM, et al., J Mol Med (Berl). 2012 May;90(5):587-95.
2. Voigt, P, et al., Genome Biol. 2011 Nov 29;12(11):133.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Bromodomain
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance