BRD3 (BD1), GST-tag

Catalog #: 31032
Size: 100 µg
$295
Species: Human
Host Species: E. coli
MW: 40.7 kDa
Genbank #: NM_007371
Tag(s): N-terminal GST-tag
a.a: 29-145
Description:
Human Bromodomain containing 3, also known as BRD3 or RING3L, GenBank Accession No. NM_007371, a.a. 29 – 145 corresponding to bromodomain 1 with N-terminal GST-tag, MW = 40.7 kDa, expressed in an E. coli expression system.
UniProt
Q15059
Synonym(s):
Bromodomain containing 3, BRD3, RING3L
Assay Conditions:

Enzyme was assayed according to BPS BRD3 (BD1) Assay Kit (Cat# 32513) protocol. BRD3 protein is incubated with biotinylated acetylhistone peptide substrate (Cat. #33000) in BRD assay buffer (Cat. #33001) in a 10 µl reaction for 1 hour at RT. AlphaLISA® (R) GSH acceptor beads (PerkinElmer) are added, followed by Alphascreen®(R) Streptavidin-conjugated donor beads (PerkinElmer). After 1 hour incubation at RT, Alphacounts are measured.

Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at –80°C.
Application(s):
Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
Reference(s):
1. Gamsjaeger, R., et al., Mol Cell Biol. 2011 Jul; 31(13): 2632-2640.
2. Lamonica, JM, et al., Proc Natl Acad Sci USA. 2011 May 31; 108(22): E159-168.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Bromodomain
Product Type:
Recombinant Protein
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