BRD2 (BD2), GST-tag

Catalog #: 31023
Size: 100 µg
$295
Species: Human
Host Species: E. coli
MW: 41.4 kDa
Genbank #: NM_005104
Tag(s): N-terminal GST-tag
a.a: 339-459
Description:
Human Bromodomain containing 2, also known as BRD2, RING3 or RNF3, GenBank Accession No. NM_005104, a.a. 65 – 187 corresponding to bromodomain 2 with N-terminal GST-tag, MW = 41.4 kDa, expressed in an E. coli expression system.
UniProt
P25440
Synonym(s):
BRD2, RING, RNF3, Bromodomain containing 2
Assay Conditions:

Assay was performed using BRD2 BD2 Inhibitor Screening Assay Kit, Cat. #32522. BRD2 protein is incubated with biotinylated acetyl-histone peptide substrate (Cat. #33000) in BRD assay buffer (Cat. #33001) in a 10 µl reaction for 1 hour at RT. AlphaLISA® ® GSH acceptor beads (PerkinElmer) are added, followed by Alphascreen®® Streptavidin-conjugated donor beads (PerkinElmer). After 1 hour incubation at RT, Alphacounts are measured.

Formulation:
50 mM Tris-HCl, pH 8.0, 138 mM NaCl, 2.7 mM KCl, 10% glycerol, 3 mM EDTA, 20 mM glutathione, and 2 mM DTT
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at –80°C.
Application(s):
Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
Reference(s):
1. Umehara, T., et al., FEBS Lett. 2010 Sep 24; 584(18): 3901-3908.
2. Shang, E., et al., J Cell Biochem. 2011 Oct; 112(10): 2784-2793.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Bromodomain
Product Type:
Recombinant Protein
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