BRD4, BD1 and BD2 (49-460)
Human, recombinant, N-terminal GST tag

Catalog #: 31044
Size: 100 µg

Description:
Human Bromodomain containing 4, also
known as BRD4, HUNK1, or MCAP,
amino-acids 49-460 (GenBank
Accession No. NM_058243) with Nterminal
GST tag, MW = 73.3 kDa,
expressed in an E. coli expression
system.

Assay Conditions: BRD4 protein is
incubated with biotinylated acetyl-histone
peptide substrate (Cat. #33000) in BRD
assay buffer (Cat. #33001) in a 10 μl
reaction for 1 hour at RT. AlphaLISA® GSH
acceptor beads (Perkin Elmer) are added,
followed by AlphaScreen® Streptavidin
-conjugated donor beads (Perkin Elmer).
After 1 hour incubation at RT, Alphacounts
are measured.

Application: 
Useful for the study of bromodomain
binding assays, screening inhibitors, and
selectivity profiling.

Formulated in: 40 mM Tris-HCl, pH 8,
110 mM NaCl, 2.2 mM KCl, 20% Glycerol
and 3 mM DTT.

Stability: >6 months at –80°C

References:
1. Rodriguez, RM, et al., J Mol Med (Berl).
2012 May;90(5):587-95.
2. Voigt, P, et al., Genome Biol. 2011 Nov
29;12(11):133.

PRODUCT DATA SHEETS
Lot 130123

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