BRD3, BD1 and BD2 (29-417)
Human, recombinant, N-terminal GST tag

Catalog #: 31035
Size: 100 µg

Description:
Human Bromodomain containing 3, also
known as BRD3 or RING3L, amino-acids
29 – 417 (GenBank Accession No.
NM_007371) with N-terminal GST tag,
MW = 70 kDa, expressed in an E. coli
expression system.

Assay Conditions: BRD3 protein is
incubated with biotinylated acetyl-histone
peptide substrate (Cat. #33000) in BRD
assay buffer (Cat. #33001) in a 10 μl
reaction for 30 min at RT. AlphaLISA® GSH
acceptor beads (Perkin Elmer) are added,
followed by AlphaScreen® Streptavidin-
conjugated donor beads (Perkin Elmer).
After 1 hour incubation at RT, Alphacounts
are measured.

Application:
Useful for the study of bromodomain
binding assays, screening inhibitors and
selectivity profiling.

Formulated in: 40 mM Tris-HCl, pH 8.0,
110 mM NaCl, 2.2 mM NaCl, and 20% glycerol.

Stability: >6 months at –80°C

References:
1. Gamsjaeger, R., et al., Mol Cell Biol.
2011 Jul; 31(13): 2632-2640.
2. Lamonica, JM, et al., Proc Natl Acad Sci
USA. 2011 May 31; 108(22): E159-168.

PRODUCT DATA SHEETS
Lot 120810-G2D

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