CREBBP Inhibitor Screening Assay Kit

Catalog #: 32607
Size: 384 reactions
$495
Description:
The CREBBP Inhibitor Screening Assay Kit is designed to measure the inhibition of CREBBP binding to its substrate. The key to the CREBBP Inhibitor Screening Assay Kit is a specific binding of the CREBBP bromodomain to the acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing CREBBP and an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.
Materials Required But Not Provided:
MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
Glutathione AlphaLISA® Acceptor Beads, 5 mg/ml (PerkinElmer #AL109C)
AlphaScreen® Streptavidin-conjugated donor beads, 5 mg/ml (PerkinElmer #6760002)
Optiplate-384 (PerkinElmer #6007290)
AlphaScreen® microplate reader
Adjustable micropipettor and sterile tips
Synonym(s):
CREBBP, CREB bindingprotein, CBP, RSTS and KAT3A, activity assay kit
Supplied As:
Kit comes in a convenient AlphaLISA® format, with biotinylated histone peptide substrate, assay buffer, detection buffer and purified GST-tagged CREBBP bromodomain to perform a total of 384 enzyme reactions.
Contraindications:
DMSO above 0.5%. Only limited amounts of DMSO can be included, as it has been shown to disrupt BRD-ligand interaction. Avoid green and blue dyes that absorb light in the Alphascreen® signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect Alphascreen® assays.
Format:

COMPONENTS:

Instructions for use:
See assay kit data sheet for detailed protocol.
Storage / Stability:

At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Application(s):
Useful for the study of bromodomain binding assays, screening bromodomain inhibitors and selectivity profiling.
Reference(s):
Filippakopoulos, P., et al., Cell 2012; 149:214.
Notes:
MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
Glutathione AlphaLISA® Acceptor Beads, 5 mg/ml (PerkinElmer #AL109C)
AlphaScreen® Streptavidin-conjugated donor beads, 5 mg/ml (PerkinElmer #6760002)
Optiplate-384 (PerkinElmer #6007290)
AlphaScreen® microplate reader
Adjustable micropipettor and sterile tips
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Bromodomain
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

32607.pdf