BRD2 (BD2) Inhibitor Screening Assay Kit
Catalog # 32522

Size: 384 reactions

DESCRIPTION: The BRD2 (BD2) Inhibitor Screening Assay Kit is designed to measure
the inhibition of BRD2 bromodomain 2 (BD2) from binding to its substrate. The BRD2
(BD2) Inhibitor Screening Assay Kit comes in a convenient AlphaLISA® format, with
biotinylated histone peptide substrate, assay buffer, detection buffer and purified, GST-tagged
BRD2 BD2 to perform a total of 384 enzyme reactions. The key to the BRD2
(BD2) Inhibitor Screening Assay Kit is the highly specific binding of the BRD2
bromodomain 2 to the acetylated histone substrate. With this kit, only three simple steps
on a microtiter plate are required. First, a sample containing BRD2 bromodomain 2 and
an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next,
acceptor beads are added, then donor beads, followed by reading the Alpha-counts.

COMPONENTS:

APPLICATIONS: Useful for the study of bromodomain binding assays, screening
inhibitors, and selectivity profiling.

STABILITY: At least one year from date of receipt when stored as directed.

REFERENCE: McBride, A.A., et al., Trends Microbiol. 2004; 12(12):527.

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