BRK, GST-tag Recombinant

Catalog #

40403

$355 *

Size: 10 µg

Qty

Bulk/Custom

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Description

Human BRK, also known as PTK6 (GenBank Accession No. NM_005975), 2-451 (end), with N-terminal GST-tag, MW=80 kDa, expressed in Sf9 insect cells via a baculovirus expression system.

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Synonyms

PTK6, FLJ42088

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Product Data Gallery

4-20% SDS-PAGE Coomassie Staining

Specific Activity

Product Info

Storage and Usage

Citations

Species

Human

Host Species/Expression System

Sf9 insect cells

Purity

≥65%

Format

Aqueous buffer solution

Formulation

40mM Tris pH 8.0, 110mM NaCl, 2.2mM KCl, 0.04% Tween-20, 20% glycerol, 3mM DTT and variable amounts of glutathione.

80 kDa

Amino Acids

2-451 (end)

Specific Activity

88 pmol/min/µg

Genbank #

NM_005975

UniProt #

Q13882

Tag(s)

N-terminal GST-tag

Background

BRK is a member of the non-receptor tyrosine kinases (PTKs) that contains an amino terminal SH3 and SH2 domain as well as the catalytic domain (1). BRK expression is low or undetectable in normal mammary tissue and benign lesions. However, approximately two-thirds of breast tumors express appreciable levels, and 27% of tumors over express BRK by fivefold or more (2).

References

1. Mitchell, PJ. et al. Oncogene. 1994 Aug,9(8):2383-90.
2. Mitchell, PJ. et al. Oncogene. 1997 Sep 18,15(12):1497-502. Erratum in: Oncogene 1998 Jul 9,17(1):129.

Storage/Stability

>6 months at -80°C

Assay Conditions

Enzyme reaction was conducted in a buffer (25 µl) containing 40 mM Tris-HCl (pH 7.5), 20 mM MgCl2, 100 µM ATP, 0.2 mg/ml poly-(Glu, Tyr), and BRK for 40 min at 30˚C. ATP reduction is detected using ADP-Glo™ Kinase Assay Platform (Promega Corporation, Madison, WI).

Instructions for Use

Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.

Applications

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Shipping Temperature

-80°C

Warnings

Avoid freeze/thaw cycles

Scientific Category

Kinase/Cytoplasmic Tyr

Regulatory Status

Research Use Only

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Datasheets/Protocols

40403.pdf

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BRK, GST-tag Recombinant

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