BRD4 (BD1) TR-FRET Assay Kit

Catalog #

32613

$575 *

Size: 384 reactions

Qty

Bulk/Custom

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*US Pricing only. For international pricing, please contact your local distributor.

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Description

The BRD4 (BD1) TR-FRET Assay Kit is designed to measure the inhibition of BRD4 (BD1) binding to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BRD4, substrate, and an inhibitor is incubated for sixty minutes. Then, the fluorescence intensity is measured using a fluorescence reader.

Need us to run inhibitor screens or profile your compounds against BRD4? Check out our Bromodomain Screening & Profiling Service.

This product has been cited 4 times.

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Synonyms

BRD4, Bromodomain, HUNK1, MCAP, BRD-4

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Product Data Gallery

Product Info

Storage and Usage

Citations4

Assay Kit Format

TR-FRET

Supplied As

Kit comes in a convenient format with purified BRD4 (BD1) protein, ligands, Tb donor, dye-labeled acceptor, assay buffer and microtiter plate to perform 384 reactions.

Materials Required But Not Supplied

MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET)
Adjustable micropipettor and sterile tips

Format

Catalog Number	Reagent	Amount	Storage
31040	BRD4, BD1 (49-170)	5 µg	-80°C	Avoid multiple freeze/thaw cycles!
33000	BET Bromodomain Ligand	50 µl	-80°C
	Non-acetylated Ligand 1	15 µl	-80°C
	Tb donor	2 x 10 µl	-20°C
	Dye-labeled acceptor	2 x 10 µl	-20°C
33012	3x BRD TR-FRET Assay Buffer 1	4 ml	-20°C
79969	White, nonbinding, low volume, microtiter plate	1	Room Temp.

UniProt #

O60885

References

Filippakopoulos, P., et al., Cell 2012; 149:214.

Storage/Stability

At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Instructions for Use

See assay kit data sheet for detailed protocol.

Applications

Useful for screening small molecular inhibitors for drug discovery and HTS applications.

Shipping Temperature

dry ice

Notes

The final concentration of DMSO should not exceed 0.5%.

Warnings

Avoid freeze/thaw cycles.

Disclaimers and Limitations

BPS Bioscience assay kits are validated using the listed components according to our specific protocol. Any deviations to kit components or protocols, such as using different proteins, cell/tissue lysates, or buffers (including using the same component from other commercial sources) will void any warranties on the performance of the assay kit and is not recommended.

End-users should immediately report any issues to [email protected] upon receipt of the kit, such as missing components, damaged vials, no dry ice, etc.

Scientific Category

Bromodomain

Regulatory Status

Research Use Only