BRD4 (BD1+BD2) TR-FRET Assay Kit

Catalog #
32612
$575 *
Size: 384 reactions
Qty
*US Pricing only. For international pricing, please contact your local distributor.
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Description

The BRD4 (BD1 + BD2) TR-FRET Assay Kit is designed to measure the inhibition of BRD4 (BD1 + BD2) binding to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BRD4, substrate, and an inhibitor is incubated for sixty minutes. Then, the fluorescence intensity is measured using a fluorescence reader.

Need us to run inhibitor screens or profile your compounds against BRD4 (BD1+BD2)? Check out our Bromodomain Screening Services.​

This product has been cited 4 times.

Synonyms
BRD4, Bromodomain, HUNK1, MCAP, BRD-4
Product Info
Storage and Usage
Citations4
Assay Kit Format
TR-FRET
Supplied As
Kit comes in a convenient format with purified BRD4 (BD1 and BD2), ligands, Tb donor, dye-labeled acceptor, assay buffer and microtiter plate to perform 384 reactions.
Materials Required But Not Supplied

Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET)
Adjustable micropipettor and sterile tips

Format
Catalog # Component Amount Storage 
31044 BRD4 (BD1+BD2)* 10 µg   -80°C Avoid
freeze/
thaw
cycles!
33000 BET Bromodomain Ligand  50 µl -80°C
33005 Non-acetylated Ligand 1 15 µl   -80°C
  Tb-labeled donor 2 x 10 µl -20°C
  Dye-labeled acceptor 2 x 10 µl -20°C
33012 3x BRD TR-FRET Assay Buffer 1  4 ml -20°C
79969 White, Nonbinding, low volume, microtiter plate  1 Room Temp

 

 

 

 

 

 

 

 

 

 

 


*The concentration of BRD4 is lot-specific and will be indicated on the tube containing the protein

UniProt #
O60885
References

Filippakopoulos, P., et al., Cell 2012; 149:214.